Anticorps Polyclonal de lapin anti-DUSP16

DUSP16 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14237-1-AP

Synonymes

DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7

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Galerie de données de validation

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  WB analysis of PC-12 using 14237-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of HeLa using 14237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of COLO 320 using 14237-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  WB analysis of mouse brain using 14237-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human colon using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-12, cellules COLO 320, cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

14237-1-AP cible DUSP16 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DUSP16 Protéine recombinante Ag5479
• Nom complet: dual specificity phosphatase 16
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC042101
• Symbole du gène: DUSP16
• Identification du gène (NCBI): 80824
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling.

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.; Authors - Ze-Qing Pu
human	WB	Int Immunopharmacol; Berberine derivative DCZ0358 induce oxidative damage by ROS-mediated JNK signaling in DLBCL cells; Authors - Qilin Feng
human,mouse	IHC	J Mol Cell Cardiol; Deficiency of smooth muscle cell ILF3 alleviates intimal hyperplasia via HMGB1 mRNA degradation-mediated regulation of the STAT3/DUSP16 axis; Authors - Ya-Min Hou
human	WB	Int J Hyperthermia; Deregulated JNK signaling enhances apoptosis during hyperthermia; Authors - Atsushi Enomoto

Produits associés

p38 MAPK Polyclonal antibody

14064-1-AP