Anticorps Polyclonal de lapin anti-cleaved-Caspase 3

cleaved-Caspase 3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

128

N° de cat : 25546-1-AP

Synonymes

cleaved-Caspase 3

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Galerie de données de validation

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  WB analysis of HepG2 using 25546-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 25546-1-AP

  Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (cleaved-caspase 3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 25546-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25546-1-AP

  Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 25546-1-AP (Caspase 3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 25546-1-AP

  IP Result of anti-Caspase 3 (IP:25546-1-AP, 4ug; Detection:25546-1-AP 1:300) with Jurkat cells lysate 3200ug.

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  IHC staining of human skeletal muscle using 25546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 25546-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 25546-1-AP

  Immunofluorescent analysis of HepG2 cells using 25546-1-AP (Caspase 3 Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 25546-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Caspase 3 antibody (25546-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HepG2 traitées à la tunicamycine, cellules HeLa traitées à la tunicamycine, cellules Jurkat traitées à la staurosporine
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 108 publications below
• IHC: See 9 publications below
• IF: See 4 publications below
• FC: See 1 publications below

Informations sur le produit

25546-1-AP cible cleaved-Caspase 3 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: caspase 3, apoptosis-related cysteine peptidase
• Masse moléculaire calculée: 32 kDa
• Poids moléculaire observé: 17 kDa
• Numéro d’acquisition GenBank: NM_032991
• Symbole du gène: CASP3
• Identification du gène (NCBI): 836
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Caspase 3, also named CPP32, SCA-1, and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7, and -9. CASP3 is involved in the cleavage of huntingtin. The antibody can recognize active-caspase 3.

Publications

Species	Application	Title
human		Nat Methods; DART-seq: an antibody-free method for global m6A detection.; Authors - Kate D Meyer
human	WB	Autophagy; Increased mitochondrial fission promotes autophagy and hepatocellular carcinoma cell survival through the ROS-modulated coordinated regulation of the NFKB and TP53 pathways.; Authors - Qichao Huang
mouse	WB	Diabetes; MICU1 Alleviates Diabetic Cardiomyopathy Through Mitochondrial Ca(2+)-dependent Antioxidant Response.; Authors - Lele Ji
		PLoS Biol; The Machado-Joseph Disease Deubiquitinase Ataxin-3 Regulates the Stability and Apoptotic Function of p53.; Authors - Hongmei Liu
human	WB	Biol Res; MicroRNA-1247 inhibits cell proliferation by directly targeting ZNF346 in childhood neuroblastoma.; Authors - Tingting Wu
rat	WB	Oxid Med Cell Longev; Classic Prescription, Kai-Xin-San, Ameliorates Alzheimer's Disease as an Effective Multitarget Treatment: From Neurotransmitter to Protein Signaling Pathway.; Authors - Sirui Guo