Anticorps Polyclonal de lapin anti-Cathepsin V

Cathepsin V Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 18442-1-AP

Synonymes

cathepsin L2, Cathepsin U, Cathepsin V, CATL2, CTSL2, CTSU, CTSV

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Galerie de données de validation

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  WB analysis using 18442-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 18442-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 18442-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18442-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18442-1-AP (Cathepsin V antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu hépatique de rat, tissu testiculaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

18442-1-AP cible Cathepsin V dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin V Protéine recombinante Ag13284
• Nom complet: cathepsin L2
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 26-36 kDa
• Numéro d’acquisition GenBank: BC023504
• Symbole du gène: Cathepsin V
• Identification du gène (NCBI): 1515
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cathepsin V is a lysosomal cysteine protease that has an important role in corneal physiology. Cathepsin V was found to be differentially expressed in relation to pigmentary skin backgrounds, and was about 7.5-folds higher in keratinocytes from lightly pigmented, relative to darkly pigmented skins. It is expressed in primary keratinocytes, melanocytes, and HaCaT keratinocytes, but not in primary fibroblasts. The purified cathepsin L2 was a high molecular weight proteinase of about 78 kDa, but it was unstable to SDS and reducing conditions, which dissociated parts of the proteinase into 66 kDa, 31 kDa and 26 kDa fragments(Food Chemistry,Volume11,Issue4,879-886) and it can form a complex of 70 kDa with an unidentified subunit(PMID:1930136).

Publications

Species	Application	Title
human	IHC	Mol Carcinog; Cathepsin V is correlated with the prognosis and tumor microenvironment in liver cancer; Authors - Junyu Liu