Anticorps Polyclonal de lapin anti-Cathepsin H

Cathepsin H Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10315-1-AP

Synonymes

ACC 4, ACC 5, Cathepsin H, CPSB, CTSH, minichain

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Galerie de données de validation

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  WB analysis using 10315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10315-1-AP (Cathepsin H antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10315-1-AP (Cathepsin H antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 10315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10315-1-AP (Cathepsin H antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 10315-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10315-1-AP (Cathepsin H antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 10315-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin H antibody (10315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HaCaT, cellules Raji, cellules U-937
• Résultats positifs en IHC: tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

10315-1-AP cible Cathepsin H dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cathepsin H Protéine recombinante Ag0361
• Nom complet: cathepsin H
• Masse moléculaire calculée: 41 kDa, 28 kDa
• Poids moléculaire observé: 28, 41 kDa
• Numéro d’acquisition GenBank: BC002479
• Symbole du gène: Cathepsin H
• Identification du gène (NCBI): 1512
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cathepsin H (CTSH, synonyms: CPSB, MGC1519, minichain) is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors.Cathepsin H is a unique member of the cysteine cathepsins that acts primarily as an aminopeptidase. Like other cysteine cathepsins, it is synthesized as an inactive precursor and activated by proteolytic removal of its propeptide. In human cells, Cathepsin H exists an autocatalytic form, proceeding from an inactive 41 kDa pro-form, through a 30 kDa intermediate form, to the 28 kDa mature form (PMID: 22704610).

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Distinct immune microenvironment of lung adenocarcinoma in never-smokers from smokers; Authors - Wenxin Luo
human	IHC	Oncogene; Cathepsin H regulated by the thyroid hormone receptors associate with tumor invasion in human hepatoma cells.; Authors - Wu S-M SM
rat	IHC	Microscopy (Oxf); Histochemical examination on principal collagen fibers in periodontal ligaments of ascorbic acid-deficient ODS-od/od rats.; Authors - Tomoka Hasegawa
rat	WB	Front Pediatr; Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.; Authors - Jun Wang
mouse	FC	Nature; Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.; Authors - Dali Han