Anticorps Polyclonal de lapin anti-CRP

CRP Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 24175-1-AP

Synonymes

C reactive protein, C-Reactive Protein, CRP, PTX1

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Galerie de données de validation

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  WB analysis using 24175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24175-1-AP (CRP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of serum from mouse injected with bacteria using 24175-1-AP

  Serum from mouse injected with bacteria were subjected to SDS PAGE followed by western blot with 24175-1-AP (CRP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 24175-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24175-1-AP (CRP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 24175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24175-1-AP (CRP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 24175-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24175-1-AP (CRP Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: rat plasma, plasma humain, sérum de souris injecté avec des bactéries
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below

Informations sur le produit

24175-1-AP cible CRP dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CRP Protéine recombinante Ag21466
• Nom complet: C-reactive protein, pentraxin-related
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC125135
• Symbole du gène: CRP
• Identification du gène (NCBI): 1401
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C-reactive protein (CRP) is an acute-phase serum protein synthesized by the liver in response to interleukin-6 (IL-6) during inflammation. The name of CRP derives from its ability to react with the C polysaccharide of Streptococcus pneumoniae. CRP is an annular, pentameric protein that belongs to the pentraxin family of proteins. CRP displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It is used mainly as a marker of inflammation.

Publications

Species	Application	Title
human, mouse	WB	J Proteome Res; Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients.; Authors - Shizhen Qin
human	WB	J Proteome Res; Measurement of Organ-Specific and Acute-Phase Blood Protein Levels in Early Lyme Disease.; Authors - Yong Zhou
human	WB	Biomed Mater; Reversing cytotoxicity of uric acid by supramolecular encapsulation with acyclic cucurbit[n]uril.; Authors - Lingguang Kong
mouse	IHC	Biochem Biophys Res Commun; In vitro and in vivo study of andrographolide nanoparticles for the treatment of Mycoplasma pneumoniae pneumonia; Authors - Liling Wang
		Exp Ther Med; TLR4 gene in the regulation of periodontitis and its molecular mechanism.; Authors - Weijuan Qi
rat	WB	ACS Omega; TMT Labeling Reveals the Effects of Exercises on the Proteomic Characteristics of the Subcutaneous Adipose Tissue of Growing High-Fat-Diet-Fed Rats; Authors - Ge Song

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