Anticorps Polyclonal de lapin anti-CEP55

• Phare
• Validé par KD/KO

CEP55 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 23891-1-AP

Synonymes

C10orf3, centrosomal protein 55kDa, Centrosomal protein of 55 kDa, CEP55, Up regulated in colon cancer 6, URCC6

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Galerie de données de validation

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  WB analysis using 23891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23891-1-AP

  WB result of CEP55 antibody (23891-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CEP55 transfected HeLa cells.

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  WB analysis using 23891-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 23891-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 23891-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 23891-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 23891-1-AP (CEP55 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 23891-1-AP

  IP result of anti-CEP55 (IP:23891-1-AP, 4ug; Detection:23891-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human testis using 23891-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23891-1-AP (CEP55 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 23891-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23891-1-AP (CEP55 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23891-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CEP55 antibody (23891-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Informations sur le produit

23891-1-AP cible CEP55 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP55 Protéine recombinante Ag20940
• Nom complet: centrosomal protein 55kDa
• Masse moléculaire calculée: 54 kDa
• Poids moléculaire observé: 47-55 kDa
• Numéro d’acquisition GenBank: BC008947
• Symbole du gène: CEP55
• Identification du gène (NCBI): 55165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEP55, also named as C10orf3 and URCC6, is a 55 kDa protein which located in the centrosome in interphase cells and recruited to the midbody during cytokinesis, is essential for completion of cell abscission. It plays a role in mitotic exit and cytokinesis.CEP55 has two isoforms with the MW of 47 and 55 kDa.

Publications

Species	Application	Title
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
human	WB,IP,IF	Cell Death Dis; iASPP-PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation.; Authors - Kun Gao ; KD Validated
human	IHC	Int J Cancer; Development and validation of a five-gene model to predict postoperative brain metastasis in operable lung adenocarcinoma.; Authors - Fangqiu Fu
human	WB	Front Oncol; USP9X Is Required to Maintain Cell Survival in Response to High-LET Radiation.; Authors - Catherine M Nickson ; KD Validated
human	WB,IHC	Cells; CEP55 Promotes Cell Motility via JAK2⁻STAT3⁻MMPs Cascade in Hepatocellular Carcinoma.; Authors - Minjing Li ; KD Validated
human	IF	iScience; Non-muscle Myosin-II Is Required for the Generation of a Constriction Site for Subsequent Abscission.; Authors - Kangji Wang

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