Cdkn2a/p16INK4a Polyclonal antibody

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  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (Cdkn2a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28416-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28416-1-AP (p16INK4a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cdkn2a/p16INK4a Polyclonal Antibody for WB, ELISA

Cat No. 28416-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

Cdkn2a, p16INK4a, Cyclin-dependent kinase inhibitor 2A, p16, p16(INK4a)

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Neuro-2a cells, RAW 264.7 cells, C6 cells, mouse uterus tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

28416-1-AP targets Cdkn2a/p16INK4a in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28927

  Product name: Recombinant mouse Cdkn2a protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-168 aa of NM_001040654

  Sequence: MESAADRLARAAAQGRVHDVRALLEAGVSPNAPNSFGRTPIQVMMMGNVHVAALLLNYGADSNCEDPTTFSRPVHDAAREGFLDTLVVLHGSGARLDVRDAWGRLPLDLAQERGHQDIVRYLRSAGCSLCSAGWSLCTAGNVAQTDGHSFSSSTPRALELRGQSQEQS

• Full Name: cyclin-dependent kinase inhibitor 2A
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 18-23 kDa
• GenBank Accession Number: NM_001040654
• Gene Symbol: Cdkn2a
• Gene ID (NCBI): 12578
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51480
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cdkn2a (cyclin-dependent kinase inhibitor 2a) is a cell cycle regulating gene encoding alternate proteins, p16Ink4a and p19Arf, that arrest the G1 phase through CDK4/6 inhibition. As they are tumor suppressors, p16Ink4a and p19Arf can be targeted for treatment of various cancers, including melanoma, cervical, and esophageal cancers. The antibody is suitable for rats and mice.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
mouse	WB	Mol Neurobiol; Hypoxic Natural Killer Cells-Derived HIF-1α-Containing Exosomes Inhibit Cellular Senescence and Apoptosis in Neurocytes to Ameliorate Alzheimer's Disease by Eliminating Oxidative Damages; Authors - Yang Zhou