Anticorps Polyclonal de lapin anti-CD86
CD86 Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, souris et plus (1)
Applications
WB, ELISA, Cell treatment, IF
Conjugaison
Non conjugué
158
N° de cat : 13395-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules Daudi, cellules Raji, cellules Ramos, cellules RAW 264.7 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 56 publications below |
| IF | See 92 publications below |
| FC | See 14 publications below |
Informations sur le produit
13395-1-AP cible CD86 dans les applications de WB, ELISA, Cell treatment, IF et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | rat, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | CD86 Protéine recombinante Ag4063 |
| Nom complet | CD86 molecule |
| Masse moléculaire calculée | 329 aa, 38 kDa |
| Poids moléculaire observé | 60-80 kDa |
| Numéro d’acquisition GenBank | BC040261 |
| Symbole du gène | CD86 |
| Identification du gène (NCBI) | 942 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
CD86 (also known as B7.2) is a costimulatory molecule belonging to the immunoglobulin superfamily. Primarily expressed on antigen-presenting cells (APCs), including B cells, dendritic cells, and macrophages, CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of CD86 with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of CD86 with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. This antibody detects a band of approximately 65-70 kDa, consistent with glycosylated form of CD86 (PMID: 8592066, 21849678).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CD86 antibody 13395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Mater Engineered Bacterial Outer Membrane Vesicles as Controllable Two-way Adaptors to Activate Macrophage Phagocytosis for Improved Tumor Immunotherapy | ||
Adv Sci (Weinh) Turmeric-Derived Nanoparticles Functionalized Aerogel Regulates Multicellular Networks to Promote Diabetic Wound Healing | ||
Acta Pharm Sin B Converting bacteria into autologous tumor vaccine via surface biomineralization of calcium carbonate for enhanced immunotherapy | ||
Adv Healthc Mater A ROS-Responsive Liposomal Composite Hydrogel Integrating Improved Mitochondrial Function and Pro-Angiogenesis for Efficient Treatment of Myocardial Infarction. | ||
Aging Cell Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells. | ||
Acta Biomater Bacterial navigation for tumor targeting and photothermally-triggered bacterial ghost transformation for spatiotemporal drug release. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Martin (Verified Customer) (09-28-2022) | Antibody is working very well
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