- Featured Product
- KD/KO Validated
MARCH8 Polyclonal antibody
MARCH8 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 14119-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue |
Positive IP detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 11 publications below |
WB | See 17 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
IP | See 2 publications below |
CoIP | See 1 publications below |
Product Information
14119-1-AP targets MARCH8 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, zebrafish |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MARCH8 fusion protein Ag5268 |
Full Name | membrane-associated ring finger (C3HC4) 8 |
Calculated Molecular Weight | 33 kDa |
Observed Molecular Weight | 30-33 kDa |
GenBank Accession Number | BC066988 |
Gene Symbol | MARCH8 |
Gene ID (NCBI) | 220972 |
RRID | AB_2140168 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MARCH8 (Membrane-associated RING finger protein 8) is also named as MIR, RNF178 and belongs to the RING zinc finger protein family. It functions as an E3 ubiquitin ligase for immune recognition-related molecules (e.g. major histocompatibility complex class I, B7-2, and ICAM-1). It plays a key role in controlling MHC class II surface expression by regulated ubiquitination of a lysine residue in the beta-chain (PMID:22761441).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for MARCH8 antibody 14119-1-AP | Download protocol |
IP protocol for MARCH8 antibody 14119-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun MARCH8 inhibits influenza A virus infection by targeting viral M2 protein for ubiquitination-dependent degradation in lysosomes. | ||
Autophagy BST2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus nucleocapsid protein with selective autophagy.
| ||
Autophagy 2AB protein of Senecavirus A antagonizes selective autophagy and type I interferon production by degrading LC3 and MARCHF8.
| ||
Proc Natl Acad Sci U S A The E3 ubiquitin ligase MARCH8 negatively regulates IL-1β-induced NF-κB activation by targeting the IL1RAP coreceptor for ubiquitination and degradation. | ||
Cancers (Basel) MARCH8 Suppresses Tumor Metastasis and Mediates Degradation of STAT3 and CD44 in Breast Cancer Cells. |