Anticorps Polyclonal de lapin anti-CD46

• Phare
• Validé par KD/KO

CD46 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 12494-1-AP

Synonymes

CD46, MCP, Membrane cofactor protein, MIC10, TLX, TRA2.10

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Galerie de données de validation

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  WB analysis using 12494-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12494-1-AP (CD46 antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 12494-1-AP

  IP result of anti-CD46 (IP:12494-1-AP, 4ug; Detection:12494-1-AP 1:1000) with A549 cells lysate 1800ug.

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12494-1-AP (CD46 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12494-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12494-1-AP (CD46 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12494-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD46 antibody (12494-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules MOLT-4
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du col de l'utérus humain, tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• FC: See 1 publications below

Informations sur le produit

12494-1-AP cible CD46 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD46 Protéine recombinante Ag3131
• Nom complet: CD46 molecule, complement regulatory protein
• Masse moléculaire calculée: 384 aa, 43 kDa
• Poids moléculaire observé: 50-56 kDa
• Numéro d’acquisition GenBank: BC030594
• Symbole du gène: CD46
• Identification du gène (NCBI): 4179
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).

Publications

Species	Application	Title
human	WB, FC	Mol Immunol; Functional characterization of two novel non-synonymous alterations in CD46 and a Q950H change in factor H found in atypical hemolytic uremic syndrome patients.; Authors - Frida C Mohlin
human	WB	Oncol Rep; Bioinformatic analysis of the membrane cofactor protein CD46 and microRNA expression in hepatocellular carcinoma.; Authors - Zejun Lu ; KD Validated
mouse	WB	Clin Exp Immunol; Mitigating placental injuries through up-regulating DAF in experimental APS mice:new mechanism of progesterone.; Authors - Yingchun Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrea (Verified Customer) (04-12-2022)

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa cells

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