Anticorps Polyclonal de lapin anti-CCT8

• Phare
• Validé par KD/KO

CCT8 Polyclonal Antibody for IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (1)

N° de cat : 12263-1-AP

Synonymes

C21orf112, CCT theta, CCT8, Cctq, D21S246, KIAA0002, PRED71, TCP 1 theta

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Galerie de données de validation

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  WB analysis using 12263-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12263-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12263-1-AP (CCT8 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12263-1-AP

  WB result of CCT8 antibody (12263-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CCT8 transfected HEK-293 cells.

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  IP experiment of Jurkat using 12263-1-AP

  IP result of anti-CCT8 (IP:12263-1-AP, 4ug; Detection:12263-1-AP 1:500) with Jurkat cells lysate 3160 ug.

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  IF Staining of SW480 using 12263-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using CCT8 antibody (12263-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of MCF-7 using 12263-1-AP

  Immunofluorescent analysis of MCF-7 cells, using CCT8 antibody 12263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules COLO 320, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules Raji, tissu rénal de souris
• Résultats positifs en IP: cellules Jurkat,
• Résultats positifs en IF: cellules SW480, cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12263-1-AP cible CCT8 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CCT8 Protéine recombinante Ag2926
• Nom complet: chaperonin containing TCP1, subunit 8 (theta)
• Masse moléculaire calculée: 548 aa, 60 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC012584
• Symbole du gène: CCT8
• Identification du gène (NCBI): 10694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CCT8, also named as C21orf112, CCTQ, KIAA0002, CCT-theta, TCP-1-theta and NY-REN-15, belongs to the TCP-1 chaperonin family. It is a molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, CCT8 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.(PMID:20080638)

Publications

Species	Application	Title
human	WB	Cell; Mechanism of orphan subunit recognition during assembly quality control; Authors - Yuichi Yagita
mouse	WB	Nat Genet; DYX1C1 is required for axonemal dynein assembly and ciliary motility.; Authors - Tarkar Aarti A
mouse	WB	Neuron; Network organization of the huntingtin proteomic interactome in mammalian brain.; Authors - Dyna I Shirasaki
human	WB	Nat Commun; Somatic increase of CCT8 mimics proteostasis of human pluripotent stem cells and extends C. elegans lifespan.; Authors - Alireza Noormohammadi
human	WB,IF	Proc Natl Acad Sci U S A; BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly.; Authors - Seo Seongjin S
human	WB	PLoS One; TDP-43 identified from a genome wide RNAi screen for SOD1 regulators.; Authors - Somalinga Balajee R BR

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joleen (Verified Customer) (06-03-2019)

CCT8 band shows up prominently with no background.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

CCT8 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:12263-1-AP)