Anticorps Polyclonal de lapin anti-CC2D1A

• Phare
• Validé par KD/KO

CC2D1A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16816-1-AP

Synonymes

CC2D1A, FREUD 1, Freud 1/Aki1, MRT3

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Galerie de données de validation

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  WB analysis of HEK-293 using 16816-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16816-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16816-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16816-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16816-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16816-1-AP (CC2D1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 16816-1-AP

  IP Result of anti-CC2D1A (IP:16816-1-AP, 4ug; Detection:16816-1-AP 1:2000) with mouse liver tissue lysate 4000ug.

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  IHC staining of human testis using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16816-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16816-1-AP (CC2D1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 16816-1-AP

  Immunofluorescent analysis of HepG2 cells, using CC2D1A antibody 16816-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below

Informations sur le produit

16816-1-AP cible CC2D1A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CC2D1A Protéine recombinante Ag10355
• Nom complet: coiled-coil and C2 domain containing 1A
• Masse moléculaire calculée: 951 aa, 104 kDa
• Poids moléculaire observé: 130 kDa, 104 kDa
• Numéro d’acquisition GenBank: BC064981
• Symbole du gène: CC2D1A
• Identification du gène (NCBI): 54862
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CC2D1A (coiled-coil and C2 domain-containing 1A), also known as Freud-1, Aki1 or TAPE (TBK1-associated protein in endolysosomes), is a evolutionary conserved protein located in different subcellular compartments, including the nucleus, centrosome, and endolysosomes. It acts as a scaffold protein that interacts with various proteins and plays diverse biological roles. Mutations in CC2D1A have been linked to nonsyndromic mental retardation (NMSR) and generate a truncated 85-kDa product. Several isoforms of CC2D1A proteins have also been described: 120 /130-kDa doublet of long isoform and 67 kDa short isoform. The short isoform of CC2D1A has been identified as the predominant isoform in rodent cells, while the long isoform is more abundant in human cells. Recently it has been reported that CC2D1A/TAPE is the first innate immune regulator implicated in both TLR and RLR signaling at a very early step.

Publications

Species	Application	Title
rat	WB	Anesthesiology; Astroglial MicroRNA-219-5p in the Ventral Tegmental Area Regulates Nociception in Rats.; Authors - Song Zhang
mouse	WB	J Biol Chem; TBK1-associated protein in endolysosomes (TAPE)/CC2D1A is a key regulator linking RIG-I-like receptors to antiviral immunity.; Authors - Chen Kuan-Ru KR ; KD Validated
mouse	WB	Neurotherapeutics; Loss of CC2D1A in Glutamatergic Neurons Results in Autistic-Like Features in Mice.; Authors - Cheng-Yi Yang
mouse	WB	J Neurosci; Conditional Deletion of CC2D1A Reduces Hippocampal Synaptic Plasticity and Impairs Cognitive Function through Rac1 Hyperactivation.; Authors - Cheng-Yi Yang