Anticorps Polyclonal de lapin anti-CAMP

CAMP Polyclonal Antibody for IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12009-1-AP

Synonymes

CAMP, CAP 18, CAP18, CRAMP, FALL 39, FALL39, hCAP 18, HSD26, LL37

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Galerie de données de validation

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  IHC staining of mouse spleen using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12009-1-AP (CAMP Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12009-1-AP (CAMP Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12009-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12009-1-AP (CAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12009-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CAMP antibody (12009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of THP-1 using 12009-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CAMP antibody (12009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en IHC: tissu splénique de souris, tissu cérébral de souris, tissu d'amygdalite humain, tissu de cancer du poumon humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules THP-1

Dilution recommandée

• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

12009-1-AP cible CAMP dans les applications de IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CAMP Protéine recombinante Ag2622
• Nom complet: cathelicidin antimicrobial peptide
• Masse moléculaire calculée: 170 aa, 19 kDa
• Numéro d’acquisition GenBank: BC055089
• Symbole du gène: CAMP
• Identification du gène (NCBI): 820
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CAMP is a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation. CAMP encodes the 18-kDa proprotein hCAP18. FALL-39 and LL-37 are the mature cathelicidin peptides. This antibody can recognize all the proprotein and cleaved species.

Publications

Species	Application	Title
		EMBO Mol Med; Gut microbiota-CRAMP axis shapes intestinal barrier function and immune responses in dietary gluten-induced enteropathy.; Authors - Zhengnan Ren
		Pharm Biol; Modified BuShenYiQi formula alleviates experimental allergic asthma in mice by negative regulation of type 2 innate lymphoid cells and CD4 + type 9 helper T cells and the VIP-VPAC2 signalling pathway; Authors - Muhua Huang
	IHC	Immunobiology; IL-33 synergistically promotes the proliferation of lung cancer cells in vitro by inducing antibacterial peptide LL-37 and proinflammatory cytokines in macrophages.; Authors - Yinting Jiang
		Res Vet Sci; Proteomic analysis of murine bone marrow derived dendritic cells in response to peste des petits ruminants virus.; Authors - Lingxia Li
		J Alzheimers Dis; Scutellarin Modulates the Microbiota-Gut-Brain Axis and Improves Cognitive Impairment in APP/PS1 Mice; Authors - Shujuan Zhang
human		Adv Sci (Weinh); Oral Immunotherapy Reshapes Intestinal Immunosuppression via Metabolic Reprogramming to Enhance Systemic Anti-Tumor Immunity; Authors - Xinran Cao

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