Anticorps Polyclonal de lapin anti-CTRP1

• Phare
• Validé par KD/KO

CTRP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12209-1-AP

Synonymes

C1QTNF1, CTRP1, GIP, ZSIG37

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Galerie de données de validation

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  WB analysis of human testis using 12209-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 12209-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12209-1-AP (CTRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12209-1-AP (CTRP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 12209-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12209-1-AP (CTRP1 antibody at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 12209-1-AP

  Immunofluorescent analysis of HepG2 cells, using C1QTNF1 antibody 12209-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

12209-1-AP cible CTRP1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CTRP1 Protéine recombinante Ag2854
• Nom complet: C1q and tumor necrosis factor related protein 1
• Masse moléculaire calculée: 281 aa, 32 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC021553
• Symbole du gène: CTRP1
• Identification du gène (NCBI): 114897
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

C1q and tumor necrosis factor related protein 1(CTRP1), also known as C1QTNF1, is a member of the CTRP superfamily. CTRP1 is a secreted glycoprotein synthesized by a variety of tissues and cells, such as adipose tissue and macrophages. CTRP1 is a paralog of adiponectin. CTRP1 plays a critical role in regulating inflammation and lipid metabolism and acts as a targeted treatment for atherosclerosis, heart failure, hypertension, NAFLD, and diabetes mellitus (PMID: 36812667).

Publications

Species	Application	Title
rat	WB	Front Cell Dev Biol; CTRP1 Attenuates Cerebral Ischemia/Reperfusion Injury via the PERK Signaling Pathway.; Authors - Huizhi Fei ; KD Validated
human	WB	Biomed Res Int; Identification of CTRP1 as a Prognostic Biomarker and Oncogene in Human Glioblastoma.; Authors - Liyan Chen ; KD Validated
human	IHC	Front Mol Biosci; High C1QTNF1 expression mediated by potential ncRNAs is associated with poor prognosis and tumor immunity in kidney renal clear cell carcinoma; Authors - Jiechuan Qiu

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