Anticorps Polyclonal de lapin anti-ARCN1

ARCN1 Polyclonal Antibody for WB, IHC, ELISA, IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 23843-1-AP

Synonymes

COPD, Delta COP, Delta coat protein, Coatomer subunit delta, archain 1

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Galerie de données de validation

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  WB analysis of HepG2 using 23843-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 23843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 23843-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 23843-1-AP (ARCN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 23843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 23843-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23843-1-AP (ARCN1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 23843-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ARCN1 antibody (23843-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HepG2, cellules Jurkat, tissu pancréatique de rat, tissu pancréatique de souris
• Résultats positifs en IHC: tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

23843-1-AP cible ARCN1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ARCN1 Protéine recombinante Ag20836
• Nom complet: archain 1
• Masse moléculaire calculée: 511 aa, 57 kDa
• Poids moléculaire observé: 57 kDa, 47 kDa
• Numéro d’acquisition GenBank: BC093636
• Symbole du gène: ARCN1
• Identification du gène (NCBI): 372
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Archain 1 (ARCN1), also known as delta-COP, is a subunit of the coat protein I (COPI) complex, which comprises one of the protein coats that mediate vesicle budding from the membrane. Protein sequences of ARCN1 are well conserved among eukaryotes and this protein may play a fundamental role in eukaryotic cell biology. It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. The gene of ARCN1 has been identified in chromosome band 11q23.3, and sequencing of cDNAs suggests that at least two forms of mRNA with alternative 5' ends are present. (PMID: 7782067; 20502676)

Publications

Species	Application	Title
human	IHC	Nature; Cell stress in cortical organoids impairs molecular subtype specification.; Authors - Aparna Bhaduri
human	WB	Nat Chem Biol; Targeting colorectal cancer with small-molecule inhibitors of ALDH1B1.; Authors - Zhiping Feng
human	IF	bioRxiv; Tropism of SARS-CoV-2 for Developing Human Cortical Astrocytes.; Authors - Madeline G Andrews
human	WB	Sci Adv; HPV is a cargo for the COPI sorting complex during virus entry; Authors - Mara C Harwood

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for WB detection

• Applications: Western Blot

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