Anticorps Polyclonal de lapin anti-AQP1

AQP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications

60

N° de cat : 20333-1-AP

Synonymes

AQP 1, AQP CHIP, AQP1, Aquaporin 1, Aquaporin CHIP, CHIP28, CO, Urine water channel

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Galerie de données de validation

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  WB analysis using 20333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 20333-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20333-1-AP (AQP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 20333-1-AP

  IP result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:40000) with mouse kidney tissue lysate 2160 ug.

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  IP experiment of mouse skeletal muscle using 20333-1-AP

  IP result of anti-AQP1 (IP:20333-1-AP, 4ug; Detection:20333-1-AP 1:1500) with mouse skeletal muscle tissue lysate 3600ug.

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  IHC staining of human kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 20333-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20333-1-AP (AQP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse kidney using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 20333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP1 antibody (20333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, muscle squelettique de rat, rein de rat, rein de souris, tissu cardiaque humain
• Résultats positifs en IP: tissu rénal de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cancer du sein humain, tissu rénal de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:3000-1:12000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IHC: See 23 publications below
• IF: See 22 publications below
• IP: See 2 publications below
• FC: See 2 publications below

Informations sur le produit

20333-1-AP cible AQP1 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AQP1 Protéine recombinante Ag14093
• Nom complet: aquaporin 1 (Colton blood group)
• Masse moléculaire calculée: 269 aa, 29 kDa
• Poids moléculaire observé: 25-28 kDa, 35-50 kDa
• Numéro d’acquisition GenBank: BC022486
• Symbole du gène: AQP1
• Identification du gène (NCBI): 358
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AQP1 is a member of aquaporins (AQPs) that are small membrane-spanning proteins facilitating water transport. AQP1 is expressed in most tissues in the mammalian body. Alterations of AQP1 expression have been linked to variety of diseases, including cancer. The predicted molecular weight of AQP1 is around 28 kDa, while highly glycosylated form can also be observed around 35-50 kDa. (PMID:20965731,16508653, 1530176).

Publications

Species	Application	Title
pig	IHC	Kidney Int; Physiologic Homeostasis after Pig-to-Human Kidney Xenotransplantation; Authors - Eric Judd
human	IF	Theranostics; Histone H3K27 methyltransferase EZH2 regulates apoptotic and inflammatory responses in sepsis-induced AKI; Authors - Bojun Li
human	IHC	Proc Natl Acad Sci U S A; Direct visualization of the arterial wall water permeability barrier using CARS microscopy.; Authors - Bertrand M Lucotte
human	IHC,IF	Cancer Res; Stromal-Based Signatures for the Classification of Gastric Cancer.; Authors - Mark T Uhlik
		Cell Rep; ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells; Authors - Danping Lu
mouse	IF	Clin Transl Med; NLRP3 inflammasome of renal tubular epithelial cells induces kidney injury in acute hemolytic transfusion reactions.; Authors - Zhixin Liu

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16473-1-AP