• Aperçu
  • Applications testées
  • Informations sur le produit
  • Publications (11)
  • Protocole
  • Produits associés (5)
  • Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-5 Lipoxygenase

5 Lipoxygenase Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 10021-1-Ig

Synonymes

ALOX5, 5LOX, 5-LO, 5LO, 5-lipoxygenase



Applications testées

Résultats positifs en WBcellules DU 145, cellules HL-60, cellules K-562, cellules PC-3, tissu placentaire humain
Résultats positifs en IF/ICCcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10021-1-Ig cible 5 Lipoxygenase dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Protéine recombinante
Nom complet arachidonate 5-lipoxygenase
Masse moléculaire calculée 674 aa, 78 kDa
Poids moléculaire observé 70-78 kDa
Numéro d’acquisition GenBankBC130332
Symbole du gène 5 Lipoxygenase
Identification du gène (NCBI) 240
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).

Protocole

Product Specific Protocols
WB protocol for 5 Lipoxygenase antibody 10021-1-IgDownload protocol
IF protocol for 5 Lipoxygenase antibody 10021-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
mouseWB,IHC

Front Pharmacol

Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway

Authors - Tong Zhou
human,mouseWB,IHC,IF

Int Immunopharmacol

The ALOX5 inhibitor Zileuton regulates tumor-associated macrophage M2 polarization by JAK/STAT and inhibits pancreatic cancer invasion and metastasis

Authors - Wei-Min Hu
mouseWB

Biochem Pharmacol

PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1.

Authors - Yunhui Xu
humanWB

J Biol Chem

Inhibition of 5-lipoxygenase selectively triggers disruption of c-Myc signaling in prostate cancer cells.

Authors - Sivalokanathan Sarveswaran
  • KD Validated
humanWB

PLoS One

MK591, a Second Generation Leukotriene Biosynthesis Inhibitor, Prevents Invasion and Induces Apoptosis in the Bone-Invading C4-2B Human Prostate Cancer Cells: Implications for the Treatment of Castration-Resistant, Bone-Metastatic Prostate Cancer.

Authors - Sivalokanathan Sarveswaran
ratIHC

Exp Cell Res

Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Authors - Fen Xu

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