- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-ALDH1A1
ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA
Conjugaison
Non conjugué
121
N° de cat : 15910-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules K-562, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris |
Résultats positifs en IP | tissu hépatique de souris, |
Résultats positifs en IHC | tissu testiculaire de souris, tissu hépatique humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF-P | tissu de côlon de souris, tissu testiculaire de souris |
Résultats positifs en IF/ICC | cellules A549, |
Résultats positifs en FC (Intra) | cellules HepG2 |
Dilution recommandée
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 103 publications below |
IHC | See 23 publications below |
IF | See 15 publications below |
ELISA | See 2 publications below |
CoIP | See 1 publications below |
Informations sur le produit
15910-1-AP cible ALDH1A1 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | rat, bovin, Humain, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | ALDH1A1 Protéine recombinante Ag8665 |
Nom complet | aldehyde dehydrogenase 1 family, member A1 |
Masse moléculaire calculée | 501 aa, 55 kDa |
Poids moléculaire observé | 50-55 kDa |
Numéro d’acquisition GenBank | BC001505 |
Symbole du gène | ALDH1A1 |
Identification du gène (NCBI) | 216 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.
Protocole
Product Specific Protocols | |
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WB protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
IHC protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
IF protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
IP protocol for ALDH1A1 antibody 15910-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders. | ||
Int J Oral Sci LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma | ||
Nat Commun TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling. | ||
J Pineal Res Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis | ||
EMBO J RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Shinford Z. (Verified Customer) (01-31-2024) | The antibody performs exceptionally well; the band on the membrane is remarkably distinct.
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FH Matthew (Verified Customer) (08-07-2023) | Antibody worked perfectly with overnight incubation at 4C. Diluted 1:1000 in TBST.
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FH Udesh (Verified Customer) (02-24-2023) | Worked well at 1:1000 in 5% BSA O/N at 4 degrees
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FH Susmita (Verified Customer) (09-17-2020) | Antibody is working good for WB
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FH Mario Armando (Verified Customer) (02-18-2020) | I used this ALDH1A1 antibody on different human and mouse tissues including adipose tissue, heart and skeletal muscle. It worked wonderfully even when using paraffin embedded sections in both human and mouse.
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FH Joleen (Verified Customer) (06-07-2019) | Prominent band correspond to ALDH1A1. Works well in western blot.
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