• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-ALDH1A1

ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

N° de cat : 15910-1-AP

Synonymes

ALDH1A 1, ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC



Applications testées

Résultats positifs en WBcellules K-562, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
Résultats positifs en IPtissu hépatique de souris,
Résultats positifs en IHCtissu testiculaire de souris, tissu hépatique humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF-Ptissu de côlon de souris, tissu testiculaire de souris
Résultats positifs en IF/ICCcellules A549,
Résultats positifs en FC (Intra)cellules HepG2

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

15910-1-AP cible ALDH1A1 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

Réactivité Humain, rat, souris
Réactivité citéerat, bovin, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène ALDH1A1 Protéine recombinante Ag8665
Nom complet aldehyde dehydrogenase 1 family, member A1
Masse moléculaire calculée 501 aa, 55 kDa
Poids moléculaire observé 50-55 kDa
Numéro d’acquisition GenBankBC001505
Symbole du gène ALDH1A1
Identification du gène (NCBI) 216
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.

Protocole

Product Specific Protocols
WB protocol for ALDH1A1 antibody 15910-1-APDownload protocol
IHC protocol for ALDH1A1 antibody 15910-1-APDownload protocol
IF protocol for ALDH1A1 antibody 15910-1-APDownload protocol
IP protocol for ALDH1A1 antibody 15910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.

Authors - Xingxing Xu
mouseWB

Mol Cell

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Authors - Nina Oberbeck
humanWB,IHC,IF

Int J Oral Sci

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma

Authors - Yuantong Liu
humanWB,IHC

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
human,mouseWB,IHC,IF

J Pineal Res

Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis

Authors - Xinyue Luo
humanIF

EMBO J

RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis.

Authors - Maria Laura Tognoli

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford Z. (Verified Customer) (01-31-2024)

The antibody performs exceptionally well; the band on the membrane is remarkably distinct.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer cell lines
ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:15910-1-AP)
FH

Matthew (Verified Customer) (08-07-2023)

Antibody worked perfectly with overnight incubation at 4C. Diluted 1:1000 in TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse mammary epithelial cell
FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS Osteosarcoma cells
ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:15910-1-AP)
FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working good for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 WB;
FH

Mario Armando (Verified Customer) (02-18-2020)

I used this ALDH1A1 antibody on different human and mouse tissues including adipose tissue, heart and skeletal muscle. It worked wonderfully even when using paraffin embedded sections in both human and mouse.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: OCT embedded frozen section of Human Adipose tissue
ALDH1A1 Antibody Immunofluorescence, validation (1:100 dilution) in OCT embedded frozen section of Human Adipose tissue (Cat no:15910-1-AP)
FH

Joleen (Verified Customer) (06-07-2019)

Prominent band correspond to ALDH1A1. Works well in western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ALDH1A1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15910-1-AP)