Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IP detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17165-1-AP targets ADH1B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10630 Product name: Recombinant human ADH1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-375 aa of BC033009 Sequence: MSTAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF Predict reactive species |
| Full Name | alcohol dehydrogenase 1B (class I), beta polypeptide |
| Calculated Molecular Weight | 375 aa, 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC033009 |
| Gene Symbol | ADH1B |
| Gene ID (NCBI) | 125 |
| RRID | AB_2878353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00325 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for ADH1B antibody 17165-1-AP | Download protocol |
| WB protocol for ADH1B antibody 17165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ADH1B antibody (Cat# 17165-1-AP) has 12 total citations across journals including Nature Communications, PNAS, Cell Death Discovery, Biomarker Research, Endocrinology, Experimental Eye Research, and others. Research fields span alcoholic liver disease, alcohol metabolism, adipogenesis, and multiple cancer types (hepatocellular, gastric, esophageal, lung adenocarcinoma, cutaneous squamous cell carcinoma), as well as ovarian follicular differentiation and metabolic reprogramming. Applications include WB, IF, IP, CoIP, and IHC.
| Species | Application | Title |
|---|---|---|
Nat Commun MG53 mediates skeletal muscle-liver cross-talk and enhances alcohol metabolism in alcoholic liver disease. | ||
Biomark Res Functional heterogeneity of cancer-associated fibroblasts with distinct neoadjuvant immunotherapy plus chemotherapy response in esophageal squamous cell carcinoma | ||
Cell Death Discov Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer. | ||
Proc Natl Acad Sci U S A ADH1B, the adipocyte-enriched alcohol dehydrogenase, plays an essential, cell-autonomous role in human adipogenesis | ||
J Ethnopharmacol Effects of Hovenia dulcis fruit and peduncle extract on alcohol metabolism | ||
Front Pharmacol Identification of afatinib-associated ADH1B and potential small-molecule drugs targeting ADH1B for hepatocellular carcinoma |
