Anticorps Polyclonal de lapin anti-P glycoprotein

P glycoprotein Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IP, CoIP, ELISA, IF

Conjugaison

Non conjugué

Publications

86

Avis (2)

N° de cat : 22336-1-AP

Synonymes

ABC20, ABCB1, CD243, CLCS, GP170, MDR1, Multidrug resistance protein 1, P glycoprotein, P glycoprotein 1, P-gp

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Galerie de données de validation

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  WB analysis using 22336-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22336-1-AP (P glycoprotein antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 22336-1-AP

  IP result of anti-P glycoprotein (IP:22336-1-AP, 4ug; Detection:22336-1-AP 1:300) with human placenta tissue lysate 4000ug.

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  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22336-1-AP (P glycoprotein antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22336-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22336-1-AP (P glycoprotein antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa non bouillies, cellules HepG2 non bouillies
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 78 publications below
• IHC: See 15 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

22336-1-AP cible P glycoprotein dans les applications de WB, IHC, IP, CoIP, ELISA, IF et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: P glycoprotein Protéine recombinante Ag17811
• Nom complet: ATP-binding cassette, sub-family B (MDR/TAP), member 1
• Masse moléculaire calculée: 1280 aa, 142 kDa
• Poids moléculaire observé: 130-150 kDa
• Numéro d’acquisition GenBank: BC130424
• Symbole du gène: P glycoprotein
• Identification du gène (NCBI): 5243
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ABCB1 (also known as P-gp/ P-glycoprotein, MDR1/multidrug-resistance 1) is a plasma membrane protein first discovered in multidrug resistant tumor cells. ABCB1 can extrude a large number of medically relevant compounds from cells and causes drug resistance. ABCB1 is expressed in a variety of human cancers as well as normal tissues including brain, and placenta. Overexpression of ABCB1 correlates with a negative prognosis in several types of cancer. For optimal WB detection with 22336-1-AP, we recommend to avoid boiling the sample after lysis.

Publications

Species	Application	Title
human	WB	Drug Resist Updat; Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer; Authors - Wei Zhang
human	WB,IHC	Mol Cancer; Hsa-miR-3178/RhoB/PI3K/Akt, a novel signaling pathway regulates ABC transporters to reverse gemcitabine resistance in pancreatic cancer.; Authors - Jianyou Gu
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	J Control Release; cRGD-targeted heparin nanoparticles for effective dual drug treatment of cisplatin-resistant ovarian cancer; Authors - Xiaomei Liang
human	WB	ACS Appl Mater Interfaces; TPGS-Galactose-Modified Polydopamine Co-delivery Nanoparticles of Nitric Oxide Donor and Doxorubicin for Targeted Chemo-Photothermal Therapy against Drug-Resistant Hepatocellular Carcinoma.; Authors - Zijing Du
human	WB	ACS Appl Mater Interfaces; PMID: 31718159; Authors - Tengfei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

SABRINA (Verified Customer) (12-07-2021)

Good antibody, but sometimes it detects also aspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: lung and breast cancer cells

P glycoprotein Antibody Western Blot validation (1:1000 dilution) in lung and breast cancer cells (Cat no:22336-1-AP)

FH

Yu Siong (Verified Customer) (03-01-2019)

It worked perfectly for my porcine brain endothelial cells!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine brain endothelial cells

P glycoprotein Antibody Western Blot, validation (1:500 dilution) in Porcine brain endothelial cells (Cat no:22336-1-AP)

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