VPS33A Polyklonaler Antikörper

VPS33A Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Kat-Nr. : 16896-1-AP

Synonyme

hVPS33A, VPS33A

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Galerie der Validierungsdaten

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  WB analysis of human brain using 16896-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16896-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 16896-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16896-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16896-1-AP (VPS33A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 16896-1-AP

  IP Result of anti-VPS33A (IP:16896-1-AP, 4ug; Detection:16896-1-AP 1:600) with HepG2 cells lysate 1600ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, HepG2-Zellen, humanes Cerebellum-Gewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 2 publications below

Produktinformation

16896-1-AP bindet in WB, IP, IF, ELISA VPS33A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS33A fusion protein Ag10422
• Vollständiger Name: vacuolar protein sorting 33 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 596 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC016617
• Gene symbol: VPS33A
• Gene ID (NCBI): 65082
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS33A is a Sec1/Munc18 family member and is a component of the homotypic fusion and protein sorting (HOPS)-tethering complex. The mammalian HOPS complex comprises six subunits (VPS11, VPS16, VPS18, VPS33A, VPS39, and VPS41) (PMID: 23901104). This complex is required for late endosome-lysosome and autophagosome-lysosome fusion (PMID: 24554770). VPS33A plays a role in vesicular transport to the lysosomal compartment.

Publikationen

Species	Application	Title
human	WB	Nat Commun; C9orf72-catalyzed GTP loading of Rab39A enables HOPS-mediated membrane tethering and fusion in mammalian autophagy; Authors - Shen Zhang
mouse	WB	J Hazard Mater; VPS41-mediated incomplete autophagy aggravates cadmium-induced apoptosis in mouse hepatocytes; Authors - Tao Wang
human	WB	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
human	WB	Autophagy; Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.; Authors - Qiuhong Shen
human	WB	J Cell Biol; The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes.; Authors - Rituraj Marwaha
human	IF	PLoS Pathog; Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication.; Authors - Aastha Sindhwani

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