VPS25 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

VPS25 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 15669-1-AP

Synonyme

hVps25, ESCRT-II complex subunit VPS25, ELL-associated protein of 20 kDa, EAP20, DERP9

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  WB analysis of A549 using 15669-1-AP

  WB result of VPS25 antibody (15669-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS25 transfected A549 cells.

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  WB analysis using 15669-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15669-1-AP

  WB result of VPS25 antibody (15669-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS25 transfected HepG2 cells.

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  WB analysis of mouse liver using 15669-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15669-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15669-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15669-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15669-1-AP (VPS25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15669-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15669-1-AP (VPS25 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15669-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using VPS25 antibody (15669-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, Maushirngewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

15669-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA VPS25 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VPS25 fusion protein Ag8134
• Vollständiger Name: vacuolar protein sorting 25 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 176 aa, 21 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC006282
• Gene symbol: VPS25
• Gene ID (NCBI): 84313
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

Publikationen

Species	Application	Title
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human,mouse	WB,IP	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang ; KD Validated
mouse	WB	Nat Commun; Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.; Authors - Luana L Scheffer
canine	WB	Curr Biol; A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.; Authors - Eva P Karasmanis
human	WB	J Cell Biol; VPS37A directs ESCRT recruitment for phagophore closure.; Authors - Yoshinori Takahashi
pig	WB,IF	PLoS Pathog; Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.; Authors - Chun-Chun Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Viviana (Verified Customer) (04-27-2022)

It works very well for WB (1:1000, KD validated) and for IP (2 ug, endogenous protein).

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse embryonic tissue isolated from wild type and KD mutantss