• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

VPRBP Monoklonaler Antikörper

VPRBP Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1A7A8

Kat-Nr. : 66392-1-Ig

Synonyme

DCAF1, HIV 1 Vpr binding protein, KIAA0800, Protein VPRBP, RIP, Vpr (HIV 1) binding protein, Vpr interacting protein, VPRBP

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of various lysates using VPRBP Monoclonal antibody (66392-1-Ig)

    WB analysis using 66392-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66392-1-Ig (VPRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using VPRBP Monoclonal antibody (66392-1-Ig)

    WB analysis of HEK-293 using 66392-1-Ig

    WB result of VPRBP antibody (66392-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells.

  • Western Blot (WB) analysis of HeLa cells using VPRBP Monoclonal antibody (66392-1-Ig)

    WB analysis of HeLa using 66392-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66392-1-Ig (VPRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VPRBP Monoclonal antibody (66392-1-Ig)

    IHC staining of human breast cancer using 66392-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66392-1-Ig (VPRBP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VPRBP Monoclonal antibody (66392-1-Ig)

    IHC staining of human breast cancer using 66392-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66392-1-Ig (VPRBP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPRBP Monoclonal antibody (66392-1-Ig)

    IF Staining of HepG2 using 66392-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using VPRBP Monoclonal antibody (66392-1-Ig)

    IF Staining of human breast cancer using 66392-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using VPRBP Monoclonal antibody (66392-1-Ig)

    IF Staining of human breast cancer using 66392-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using VPRBP antibody (66392-1-Ig, Clone: 1A7A8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of K-562 cells using VPRBP Monoclonal antibody (66392-1-Ig)

    FC experiment of K-562 using 66392-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human VPRBP (66392-1-Ig, Clone:1A7A8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, DU 145-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, PC-3-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IFHepG2-Zellen, humanes Mammakarzinomgewebe
Erfolgreiche Detektion in FCK-562-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)IF : 1:400-1:1600
Durchflusszytometrie (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

KD/KOSee 1 publications below
WBSee 1 publications below

Produktinformation

66392-1-Ig bindet in WB, IHC, IF, FC, ELISA VPRBP und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen VPRBP fusion protein Ag2184
Vollständiger Name Vpr (HIV-1) binding protein
Berechnetes Molekulargewicht 1506 aa, 169 kDa
Beobachtetes Molekulargewicht 169 kDa
GenBank-ZugangsnummerBC022792
Gene symbol VPRBP
Gene ID (NCBI) 9730
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Protokolle

Produktspezifische Protokolle
WB protocol for VPRBP antibody 66392-1-IgProtokoll herunterladen
IHC protocol for VPRBP antibody 66392-1-IgProtokoll herunterladen
IF protocol for VPRBP antibody 66392-1-IgProtokoll herunterladen
FC protocol for VPRBP antibody 66392-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
human,mouseWB

Nat Commun

Phosphorylation and stabilization of EZH2 by DCAF1/VprBP trigger aberrant gene silencing in colon cancer

Authors - Nikhil B Ghate
  • KD Validated
View Article

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Nikhil (Verified Customer) (01-07-2022)

SW620 cell xenograft. Red colour is VprBP. Blue is DAPI.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: SW620
VPRBP Antibody Immunofluorescence validation (1:200 dilution) in SW620 (Cat no:66392-1-Ig)