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Uromodulin Polyklonaler Antikörper

Uromodulin Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11911-1-AP

Synonyme

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, uromodulin

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of human urine sample using Uromodulin Polyclonal antibody (11911-1-AP)

    WB analysis of human urine using 11911-1-AP

    human urine sample were subjected to SDS PAGE followed by western blot with 11911-1-AP (Uromodulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Uromodulin Polyclonal antibody (11911-1-AP)

    WB analysis using 11911-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11911-1-AP (Uromodulin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uromodulin Polyclonal antibody (11911-1-AP)

    IHC staining of human kidney using 11911-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11911-1-AP (Uromodulin antibody) at dilution of 1:1000 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using Uromodulin Polyclonal antibody (11911-1-AP)

    IHC staining of human kidney using 11911-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11911-1-AP (Uromodulin antibody) at dilution of 1:1000 (under 40x lens).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBhumane Urinprobe, Mausnierengewebe, Rattennierengewebe
Erfolgreiche Detektion in IHChumanes Nierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunhistochemie (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

IHCSee 2 publications below
IFSee 1 publications below

Produktinformation

11911-1-AP bindet in WB, IF, IHC, ELISA Uromodulin und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Uromodulin fusion protein Ag2494
Vollständiger Name uromodulin
Berechnetes Molekulargewicht 640 aa, 70 kDa
Beobachtetes Molekulargewicht90-105 kDa, 55-75 kDa
GenBank-ZugangsnummerBC035975
Gene symbol Uromodulin
Gene ID (NCBI) 7369
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin has been linked to water/electrolyte balance and to kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)

Protokolle

Produktspezifische Protokolle
WB protocol for Uromodulin antibody 11911-1-APProtokoll herunterladen
IHC protocol for Uromodulin antibody 11911-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
IF

Sci China Life Sci

Identification and characterization of two morphologically distinct stem cell subpopulations from human urine samples.

Authors - An-Jing Chen
View Article
humanIHC

Kidney Int Rep

Vancomycin-Associated Tubular Casts and Vancomycin Nephrotoxicity.

Authors - Ngoentra Tantranont
View Article
humanIHC

Nephrology (Carlton)

Renal expression of trefoil factor 3 mRNA in association with tubulointerstitial fibrosis in IgA nephropathy.

Authors - Keiko Tanaka
View Article