TGF Beta 1 Polyklonaler Antikörper
TGF Beta 1 Polyklonal Antikörper für IHC, WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte und mehr (2)
Anwendung
WB, IHC, IF, Cell treatment, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 18978-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | MCF-7-Zellen |
| Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 59 publications below |
| IHC | See 25 publications below |
| IF | See 15 publications below |
Produktinformation
18978-1-AP bindet in WB, IHC, IF, Cell treatment, ELISA TGF Beta 1 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Hausschwein, Hund, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | transforming growth factor, beta 1 |
| Berechnetes Molekulargewicht | 44 kDa |
| Beobachtetes Molekulargewicht | 25 kDa |
| GenBank-Zugangsnummer | NM_000660 |
| Gene symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1). Full length, inactive 44 kD TGFB1 is cleaved into mature TGFB1 (13 kD). TGFB1 also homodimerizes and heterodimerizes with TGFB2, so there is potential for multiple different band sizes in WB (12, 25, 45 to 65 kDa).
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for TGF Beta 1 antibody 18978-1-AP | Protokoll herunterladen |
| IHC protocol for TGF Beta 1 antibody 18978-1-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Circulation Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation. | ||
Biomaterials A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration | ||
Br J Dermatol Intense Foxp3(+) CD25(+) regulatory T cell infiltration is associated with high-grade cutaneous squamous cell carcinoma and counterbalanced by CD8(+) /Foxp3(+) CD25(+) ratio. | ||
Proc Natl Acad Sci U S A miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression. | ||
