TAF12 Polyklonaler Antikörper

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• KD/KO Validated

TAF12 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12353-1-AP

Synonyme

TAF12, TAF15, TAF2J, TAFII 20/TAFII 15, TAFII20, TAFII20/TAFII15

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 12353-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12353-1-AP (TAF12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of Y79 using 12353-1-AP

  IP result of anti-TAF12 (IP:12353-1-AP, 4ug; Detection:12353-1-AP 1:300) with Y79 cells lysate 2000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen
• Erfolgreiche IP: Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

12353-1-AP bindet in WB, IHC, IP, ChIP, ELISA TAF12 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TAF12 fusion protein Ag3017
• Vollständiger Name: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 21 kDa
• GenBank-Zugangsnummer: BC011986
• Gene symbol: TAF12
• Gene ID (NCBI): 6883
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation. Observed MW of TAF12 is from 14-17 kDa and 17-21 kDa.

Publikationen

Species	Application	Title
mouse	WB	Cancer Cell; A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.; Authors - Yali Xu ; KD Validated
mouse	WB	Cell Metab; Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease.; Authors - Noriyoshi Kurihara
mouse	ChIP,WB	J Bone Miner Res; Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease.; Authors - Jumpei Teramachi
mouse, human	WB	J Bone Miner Res; TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation.; Authors - Sun Quanhong Q
mouse	WB	J Bone Miner Res; Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone.; Authors - Jumpei Teramachi
human	WB	Mol Cell Biol; The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells.; Authors - Gegonne Anne A

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