STXBP2 Polyklonaler Antikörper

STXBP2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 15312-1-AP

Synonyme

Hunc18b, MUNC18 2, pp10122, STXBP2, STXBP2 protein, syntaxin binding protein 2, UNC18 2, UNC18B

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 15312-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15312-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 15312-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15312-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15312-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15312-1-AP (STXBP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15312-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15312-1-AP (STXBP2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, Jurkat-Zellen, Maushirngewebe, Mausnierengewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

15312-1-AP bindet in WB, IHC, ELISA STXBP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STXBP2 fusion protein Ag7537
• Vollständiger Name: syntaxin binding protein 2
• Berechnetes Molekulargewicht: 593 aa, 66 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: BC002869
• Gene symbol: STXBP2
• Gene ID (NCBI): 6813
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syntaxin-binding protein 2 (STXBP2, also known as Unc18-2) belongs to the STXBP/unc-18/SEC1 family. STXBP2 is involved in intracellular trafficking, control of SNARE (soluble NSF attachment protein receptor) complex assembly, and the release of cytotoxic granules by natural killer cells. Mutations in the STXBP2 gene are associated with familial hemophagocytic lymphohistiocytosis.

Publikationen

Species	Application	Title
human	WB	J Exp Med; Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency.; Authors - Frank Cichocki
human	WB	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar
human	WB	Front Immunol; An N-Terminal Missense Mutation in STX11 Causative of FHL4 Abrogates Syntaxin-11 Binding to Munc18-2.; Authors - Martha-Lena Müller
rat	WB	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
	WB	Int J Hematol; Molecular analysis of the novel L243R mutation in STXBP2 reveals impairment of degranulation activity.; Authors - Laura Viñas-Giménez

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