STRADB Polyklonaler Antikörper

STRADB Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 10688-1-AP

Synonyme

ALS2CR2, CALS 21, ILP interacting protein, ILPIP, ILPIPA, PAPK, PRO1038, Pseudokinase ALS2CR2, STRAD beta, STRADB

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Galerie der Validierungsdaten

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  WB analysis of Raji using 10688-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  WB analysis of mouse heart using 10688-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10688-1-AP (STRADB Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

• 

  IHC staining of human heart using 10688-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 10688-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10688-1-AP (STRADB Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10688-1-AP bindet in WB, IHC, ELISA STRADB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: STRADB fusion protein Ag1040
• Vollständiger Name: STE20-related kinase adaptor beta
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 28-31 kDa, 38-42 kDa
• GenBank-Zugangsnummer: BC008302
• Gene symbol: STRADB
• Gene ID (NCBI): 55437
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The 418 amino acid STRADB protein, which is also named as ALS2CR2 and ILPIP, contains a putative N-terminal ATP-binding site, a protein kinase domain, and several C-terminal phosphorylation sites. STRADB potentiates the antiapoptotic activity of XIAP by enhancing XIAP-mediated activation of JNK1 and other JNK family members, but not by modulating XIAP-mediated caspase inhibition. This protein has 3 isoforms produced by alternative splicing with the MW of 47 kDa, 42 kDa and 31 kDa.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size. No additional background bands were observed around the size range. AWESOME!

• Applications: Western Blot,
• Cell Tissue Type: 293T, U2OS

STRADB Antibody Western Blot, validation ( dilution) in 293T, U2OS (Cat no:10688-1-AP)