STAT3 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

STAT3 Monoklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

62

CloneNo.

3G2D12

Kat-Nr. : 60199-1-Ig

Synonyme

Acute phase response factor, APRF, HIES, STAT3

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 60199-1-Ig

  WB result of STAT3 antibody (60199-1-Ig, 1:3000) with si-Control and si-STAT3 transfected HeLa cells.

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  WB analysis of HeLa using 60199-1-Ig

  Western blot analysis of STAT3 in various cell lines using Proteintech antibody 60199-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60199-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 60199-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60199-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60199-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60199-1-Ig (STAT3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60199-1-Ig

  IP Result of anti-STAT3 (IP:60199-1-Ig, 5ug; Detection:60199-1-Ig 1:2000) with HeLa cells lysate 1400ug.

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  IHC staining of human cervical cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human lung cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human lung cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human heart using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human endometrial cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human endometrial cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 60199-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 60199-1-Ig

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60199-1-Ig(STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of HepG2 using 60199-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT3 antibody (60199-1-Ig, Clone: 3G2D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of HepG2 using 60199-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human STAT3 (60199-1-Ig, Clone:3G2D12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Jurkat-Zellen, RAW 264.7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Endometriumkarzinomgewebe, humanes Herzgewebe, humanes Lebergewebe, humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 56 publications below
• IHC: See 7 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

60199-1-Ig bindet in WB, IP, IHC, IF, FC, CoIP, ELISA STAT3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Ratte, Zebrafisch
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: STAT3 fusion protein Ag0360
• Vollständiger Name: signal transducer and activator of transcription 3 (acute-phase response factor)
• Berechnetes Molekulargewicht: 770 aa, 88 kDa
• Beobachtetes Molekulargewicht: 70-88 kDa
• GenBank-Zugangsnummer: BC000627
• Gene symbol: STAT3
• Gene ID (NCBI): 6774
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	ACS Nano; Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy.; Authors - Mengshi Jiang
human	WB	J Immunother Cancer; Switch receptor T3/28 improves long-term persistence and antitumor efficacy of CAR-T cells; Authors - Songbo Zhao
mouse	WB	Theranostics; Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.; Authors - Jiqian Zhang
mouse	WB	Clin Transl Med; PRKAR2A-derived circular RNAs promote the malignant transformation of colitis and distinguish patients with colitis-associated colorectal cancer.; Authors - Daiwei Wan
human	WB,IF	J Invest Dermatol; Keratinocyte growth factor-2 regulates signal-transducing adaptor protein-2-mediated signal transducer and activator of transcription 3 signaling and reduces skin scar formation.; Authors - Qingde Zhou
rat	WB	Cell Biol Toxicol; Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways; Authors - Hongyan Wang

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