SPPL2A Polyklonaler Antikörper

SPPL2A Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13255-1-AP

Synonyme

IMP 3, IMP3, Intramembrane protease 3, Presenilin like protein 2, PSL2, SPP like 2A, SPPL2A

---

Galerie der Validierungsdaten

• 

  WB analysis of human colon using 13255-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13255-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 13255-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13255-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13255-1-AP (SPPL2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13255-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13255-1-AP (SPPL2A antibody) at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Kolongewebe, Jurkat-Zellen, Maus-Kolongewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13255-1-AP bindet in WB, IHC,ELISA SPPL2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SPPL2A fusion protein Ag3935
• Vollständiger Name: signal peptide peptidase-like 2A
• Berechnetes Molekulargewicht: 520 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC025740
• Gene symbol: SPPL2A
• Gene ID (NCBI): 84888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SPPL2A(Signal peptide peptidase-like 2A) is also named as IMP3(Intramembrane protease 3), PSL2(Presenilin-like protein 2) and belongs to the peptidase A22B family. It is an aspartyl intramembrane protease, has been implicated in the proteolysis of TNF-alpha, Fas Ligand and Bri2 and endogenous signal-peptide-peptidase-like2A (SPPL2a) is localised in lysosomes/late endosomes(PMID:21896273). This protein has a signal peptide with 25 amino acid and seven glycosylation sites.