SNX27 Polyklonaler Antikörper

SNX27 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 16329-1-AP

Synonyme

KIAA0488, MRT1, MY014, SNX27, Sorting nexin 27, sorting nexin family member 27

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 16329-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16329-1-AP (SNX27 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 16329-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16329-1-AP (SNX27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human cervical cancer using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 16329-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16329-1-AP (SNX27 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

16329-1-AP bindet in WB, IHC, ELISA SNX27 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SNX27 fusion protein Ag9447
• Vollständiger Name: sorting nexin family member 27
• Berechnetes Molekulargewicht: 61 kDa, 60 kDa, 52 kDa, 28 kDa
• Beobachtetes Molekulargewicht: 52-60 kDa
• GenBank-Zugangsnummer: BC012184
• Gene symbol: SNX27
• Gene ID (NCBI): 81609
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sorting nexin 27 (SNX27), a PDZ domain-containing protein, belongs to the sorting nexin family of proteins. SNX27 promotes recycling of internalized transmembrane proteins from endosomes to the plasma membrane by linking PDZ-dependent cargo recognition to retromer-mediated transport (PMID: 21602791; 23563491). It has been shown that SNX27 deficiency contributes to the synaptic and cognitive deficits by modulating glutamate receptor recycling in Down's syndrome (PMID: 23524343). SNX27 has also been proposed to have a role in regulating β-amyloid (Aβ) generation by modulating γ-secretase activity, which is a molecular mechanism for Aβ-dependent pathogenesis in both Down's syndrome and Down's syndrome (PMID: 25437557).

Publikationen

Species	Application	Title
human	WB	J Cell Biol; Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.; Authors - Boris Simonetti
human	WB	Cells; Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells; Authors - Jiahe Lu
human	WB	bioRxiv; Five inhibitory receptors display distinct vesicular distributions in T cells; Authors - Jiahe Lu
human	WB	Cell Rep; Regulation of NMDA receptor trafficking and gating by activity-dependent CaMKIIα phosphorylation of the GluN2A subunit.; Authors - Xuan Ling Hilary Yong
human,mouse	WB	Endocrinol Diabetes Metab; Diabetes is accompanied by changes in the levels of proteins involved in endosomal GLUT4 trafficking in obese human skeletal muscle; Authors - Rachel Livingstone

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