SLCO1B3 Monoklonaler Antikörper

SLCO1B3 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

1D9A4

Kat-Nr. : 66381-1-Ig

Synonyme

LST 2, LST 3TM13, LST2, LST3, OATP 8, OATP1B3, OATP8, Organic anion transporter 8, SLC21A8, SLCO1B3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 66381-1-Ig

  Western blot analysis of SLCO1B3 in various cell lines using Proteintech antibody 66381-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 66381-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66381-1-Ig (SLCO1B3 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66381-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SLCO1B3 antibody (66381-1-Ig, Clone: 1D9A4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, BxPC-3-Zellen, Caco-2-Zellen, COLO 320-Zellen, L02-Zellen, PC-3-Zellen, SMMC-7721-Zellen, SW 1990-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

66381-1-Ig bindet in WB, IHC, IF, ELISA SLCO1B3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLCO1B3 fusion protein Ag19033
• Vollständiger Name: solute carrier organic anion transporter family, member 1B3
• Berechnetes Molekulargewicht: 702 aa, 77 kDa
• Beobachtetes Molekulargewicht: 68-77 kDa
• GenBank-Zugangsnummer: BC105597
• Gene symbol: SLCO1B3
• Gene ID (NCBI): 28234
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLCO1B3, also known as OATP8 or OATP1B3, is a member of Organic Anion Transporting Polypeptides (OATPs) which are sodium-independent organic anion transporters mediating the uptake of a wide range of structurally diverse endogenous and exogenous compounds including bile acids, hormone conjugates, peptides, toxins, as well as a multitude of therapeutic drugs. SLCO1B3 is specifically expressed in liver under normal condition, while its expression has also been observed in cancer tissues like colon, prostate and pancreas. It corresponds to Oatp1b2 in mice, with the gene symbol Slco1b2. The mouse genome encodes only a single transporter gene in the OATP1B subfamily, because SLCO1B1 and SLCO1B3 arose in primates by gene duplication after divergence from rodents. The molecular mass of SLCO1B3 is highly dependent on the glycosylation modification (deglycosylated form of 65 kDa, fully-glycosylated form of 120 kDa).

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); SLCO1B3 promotes colorectal cancer tumorigenesis and metastasis through STAT3.; Authors - Lianghui Zhi
human	WB	Nat Commun; Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity; Authors - Bei Wang
human	WB	Bull Exp Biol Med; The Effect of Original Russian Neurotropic Drugs on Organic Anion Transporting Polypeptides OATP1B1 and OATP1B3; Authors - P D Erokhina
	WB	Sci Rep; OATP1B3 (699G>A) and CYP2C9*2, *3 significantly influenced the transport and metabolism of glibenclamide and glipizide.; Authors - Fayou Yang