DAT Polyklonaler Antikörper

DAT Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Kat-Nr. : 22524-1-AP

Synonyme

DA transporter, DAT, DAT1, SLC6A3

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Galerie der Validierungsdaten

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  WB analysis using 22524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22524-1-AP

  IP sample and control were subjected to SDS PAGE followed by western blot with 22524-1-AP (DAT antibody) at dilution of 1:500. 4000ug mouse brain tissue lysate and 4ug antibodies were mixed in the IP experiment.

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  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22524-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22524-1-AP (DAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 22524-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DAT antibody (22524-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 32 publications below
• IHC: See 3 publications below
• IF: See 10 publications below

Produktinformation

22524-1-AP bindet in WB, IP, IHC, IF, ELISA DAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: DAT fusion protein Ag18297
• Vollständiger Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3
• Berechnetes Molekulargewicht: 620 aa, 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC133003
• Gene symbol: SLC6A3
• Gene ID (NCBI): 6531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DAT, also name as SLC6A3, is dopamine transporter which is a member of the sodium- and chloride- dependent neurotransmitter transporter family. Dopamine(DA) released from neurons is cleared by the DA transporter (DAT). Altered dopaminergic signaling is linked to multiple neuropsychiatric disorders, such as attention deficit hyperactive disorder, mood disorders, schizophrenia, autism and so on (PMID:30755521). 22524-1-AP can detect 70~80kDa band, which is consistent with the report (PMID:12746456).

Publikationen

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
mouse	WB	Mov Disord; Fine Particulate Matter Triggers α-Synuclein Fibrillization and Parkinson-like Neurodegeneration; Authors - Xin Yuan
rat	WB	J Affect Disord; Dysregulation of striatal dopamine D2/D3 receptor-mediated by hypocretin induces depressive behaviors in rats; Authors - Bing Chen
human	WB	Br J Pharmacol; Andrographolide alleviates parkinsonism in MPTP-PD mice via targeting DRP1-mediated mitochondrial fission.; Authors - Ji Geng
rat	IF	Brain Pathol; Reconstituting neurovascular unit with primary neural stem cells and brain microvascular endothelial cells in three-dimensional matrix.; Authors - Hongjin Wang
		Aging (Albany NY); The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling.; Authors - Zhu Zhu

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