Sodium iodide symporter Polyklonaler Antikörper

Sodium iodide symporter Polyklonal Antikörper für FC, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

40

Rezensionen (1)

Kat-Nr. : 24324-1-AP

Synonyme

NIS, SLC5A5, Sodium iodide symporter, Sodium/iodide cotransporter

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Galerie der Validierungsdaten

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  WB analysis using 24324-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse stomach using 24324-1-AP

  mouse stomach tissue were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 24324-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse testis using 24324-1-AP

  IP Result of anti-Sodium iodide symporter (IP:24324-1-AP, 4ug; Detection:24324-1-AP 1:500) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human thyroid cancer using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human thyroid cancer using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 24324-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 24324-1-AP (Sodium iodide symporter antibody at dilution of 1:50 (under 40x lens).

• 

  FC experiment of MCF-7 using 24324-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug Sodium iodide symporter antibody (24324-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:100.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Maus-Magengewebe, SGC-7901-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IHC: humanes Schilddrüsenkarzinomgewebe, humanes Eierstockgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 25 publications below
• IHC: See 5 publications below
• IF: See 13 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

24324-1-AP bindet in WB, IP, IHC, IF, FC, ChIP, ELISA Sodium iodide symporter und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Sodium iodide symporter fusion protein Ag19504
• Vollständiger Name: solute carrier family 5 (sodium iodide symporter), member 5
• Berechnetes Molekulargewicht: 643 aa, 69 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa, 75-100 kDa
• GenBank-Zugangsnummer: BC105047
• Gene symbol: SLC5A5
• Gene ID (NCBI): 6528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The sodium iodide symporter (Na+/I - symporter, NIS), encoded by SLC5A5, is an integral plasma membrane glycoprotein that plays an important role in iodide uptake by thyroid cells. Expression of sodium iodide symporter has also been found in extra-thyroidal tissues, including gastric mucosa, lactating mammary gland and salivary glands. Increased expression of sodium iodide symporter has been found in thyroid tissue from patients with Graves' disease as well as papillary thyroid carcinomas. In addition, sodium iodide symporter was found to express in majority of breast cancer tissue but not in normal tissue. Sodium iodide symporter can be a promising diagnostic and therapeutic tool for thyroid cancer and breast cancer. This antibody recognizes the mature approximately 75-100 kDa protein and a partially glycosylated 50-55 kDa protein. (PMID: 12588808, 9525971)

Publikationen

Species	Application	Title
		Adv Sci (Weinh); Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice; Authors - Xue-Jiao Tian
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
human	WB	Cell Death Differ; CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation.; Authors - Yina Liao
human	WB	Thyroid; Dimerization of the sodium/iodide symporter (NIS).; Authors - Rebecca J Thompson
human	WB	Cell Chem Biol; Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.; Authors - Martin L Read
human	IF	Cancer Lett; IGF2BP2-dependent activation of ERBB2 signaling contributes to acquired resistance to tyrosine kinase inhibitor in differentiation therapy of radioiodine-refractory papillary thyroid cancer.; Authors - Ri Sa

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Katie (Verified Customer) (08-22-2019)

Highly specific antibody for the sodium iodide symporter (NIS) with no background bands in Western blot, used at 1:1000 dilution. Very clear result. Good value for money, and excellent communication prior to delivery receipt.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Thyroid carcinoma cell lines

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