Septin 7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Septin 7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA, PLA

Konjugation

Unkonjugiert

Publikationen

26

Rezensionen (2)

Kat-Nr. : 13818-1-AP

Synonyme

septin7, SEPT7A, SEPT7, SEPT 7, CDC3

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Galerie der Validierungsdaten

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  WB analysis using 13818-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13818-1-AP (SEPT7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13818-1-AP

  WB result of Septin 7 antibody (13818-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Septin 7 transfected HepG2 cells.

• 

  WB analysis of mouse testis using 13818-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13818-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 13818-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 13818-1-AP (Septin 7 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13818-1-AP (Septin 7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung cancer using 13818-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 13818-1-AP (Septin 7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of hTERT-RPE cells using 13818-1-AP

  IF result of anti-Septin 7 (13818-1-AP, 1:1000) with hTERT-RPE cell by Dr. Moshe Kim. Green: Septin 7. Blue: DNA. 1% PFA fixed.

• 

  IF Staining of HepG2 using 13818-1-AP

  Immunofluorescent analysis of HepG2 cells, using Septin 7 antibody 13818-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, HepG2-Zellen, Jurkat-Zellen, Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 16 publications below
• IF: See 16 publications below
• CoIP: See 1 publications below

Produktinformation

13818-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA, PLA Septin 7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 7 fusion protein Ag4907
• Vollständiger Name: septin 7
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC025987
• Gene symbol: Septin 7
• Gene ID (NCBI): 989
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Septins (SEPTs) are members of a conserved family of cytoskeletal GTPases involved in various cellular processes, including cytoskeleton organization, cytokinesis, and membrane dynamics. SEPT7 is a unique septin required for filament formation. It has also been reported to be involved in migration and invasion in various cancer cells, including breast cancer and glioma.

Publikationen

Species	Application	Title
		Nat Commun; Still and rotating myosin clusters determine cytokinetic ring constriction.; Authors - Viktoria Wollrab
human	WB	J Cell Biol; The septin cytoskeleton regulates natural killer cell lytic granule release.; Authors - Prasad V Phatarpekar ; KD Validated
mouse	WB,IF	Cell Rep; Cdc42EP3/BORG2 and Septin Network Enables Mechano-transduction and the Emergence of Cancer-Associated Fibroblasts.; Authors - Fernando Calvo
mouse	IF,PLA	EMBO Rep; Cdc42-Borg4-Septin7 axis regulates HSC polarity and function; Authors - Ravinder Kandi
human	WB,IF	MBio; Role of Endothelial Cell Septin 7 in the Endocytosis of Candida albicans.; Authors - Quynh T Phan ; KD Validated
bovine	IF	Development; Sperm-borne miR-202 targets SEPT7 and regulates first cleavage of bovine embryos via cytoskeletal remodeling.; Authors - Mengyun Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amaury (Verified Customer) (03-29-2022)

I performed immunolabelling on K562. Used ice-cold methanol to permeabilize the cells and obtain very nice labelling

• Applications: Immunofluorescence
• Cell Tissue Type: K562

Septin 7 Antibody Immunofluorescence validation ( dilution) in K562 (Cat no:13818-1-AP)

FH

stephen (Verified Customer) (02-19-2019)

HeLa cells fixed with 4%PFA and permblised with 0.3% triton x100 for 5 mins. stained with septin 7ab 1:300. (green) and DAPI to mark DNA (blue). non dividing interphase cell (top). Dividing cell in anaphase (bottom)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: HeLa cells

Septin 7 Antibody Immunofluorescence, validation (1:300 dilution) in HeLa cells (Cat no:13818-1-AP)

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