CD138/Syndecan-1 Polyklonaler Antikörper

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• KD/KO Validated

CD138/Syndecan-1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

32

Kat-Nr. : 10593-1-AP

Synonyme

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1

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Galerie der Validierungsdaten

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  WB analysis using 10593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 10593-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10593-1-AP (CD138/Syndecan-1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10593-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10593-1-AP (CD138/Syndecan-1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, Mausmilzgewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:800-1:3200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 12 publications below
• IF: See 7 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

10593-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA CD138/Syndecan-1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD138/Syndecan-1 fusion protein Ag0823
• Vollständiger Name: syndecan 1
• Berechnetes Molekulargewicht: 32.5 kDa
• Beobachtetes Molekulargewicht: 60-100 kDa, 130 kDa
• GenBank-Zugangsnummer: BC008765
• Gene symbol: CD138
• Gene ID (NCBI): 6382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

Publikationen

Species	Application	Title
human	IF	Cell; Tumor-reactive antibodies evolve from non-binding and autoreactive precursors.; Authors - Roei D Mazor
human	WB,IP	Autophagy; SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes; Authors - Wang Zheng
mouse	IHC	Leukemia; miR-21 antagonism abrogates Th17 tumor promoting functions in multiple myeloma.; Authors - Marco Rossi
mouse	IF	Theranostics; Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.; Authors - Xin Chen
		Mol Neurodegener; Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model.; Authors - Yi-Ling Liu
human	WB	Cell Mol Life Sci; The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium.; Authors - Li-Na Wang

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