OXCT1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

OXCT1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

44

Rezensionen (1)

Kat-Nr. : 12175-1-AP

Synonyme

Somatic-type succinyl-CoA:3-oxoacid CoA-transferase, SCOT, OXCT, EC:2.8.3.5, 3-oxoacid CoA-transferase 1

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Galerie der Validierungsdaten

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  WB analysis using 12175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12175-1-AP (OXCT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12175-1-AP

  WB result of OXCT1 antibody (12175-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

• 

  IP experiment of mouse brain using 12175-1-AP

  IP result of anti-OXCT1 (IP:12175-1-AP, 3ug; Detection:12175-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12175-1-AP (OXCT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12175-1-AP (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (12175-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 12175-1-AP

  Immunofluorescent analysis of MCF-7 cells, using OXCT1 antibody 12175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 12175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (12175-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Prostatakarzinomgewebe, Mausherzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 40 publications below
• IHC: See 3 publications below
• IF: See 8 publications below

Produktinformation

12175-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA OXCT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: OXCT1 fusion protein Ag2818
• Vollständiger Name: 3-oxoacid CoA transferase 1
• Berechnetes Molekulargewicht: 520 aa, 56 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC009001
• Gene symbol: OXCT1
• Gene ID (NCBI): 5019
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Publikationen

Species	Application	Title
mouse	WB	Nat Med; PMID: 25686106; Authors - Yun-Hee Youm
mouse	WB	Cell Metab; PMID: 32275862; Authors - Rami Al Batran ; KD Validated
mouse	WB,IF	Cell Metab; Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.; Authors - Patrycja Puchalska ; KO Validated
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
mouse	WB,IF	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
mouse	WB	Sci Immunol; Ketogenic diet activates protective γδ T cell responses against influenza virus infection.; Authors - Emily L Goldberg

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kerry (Verified Customer) (09-26-2019)

Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.

• Applications: Immunohistochemistry,
• Cell Tissue Type: mouse

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