Pyruvate Carboxylase Polyclonal antibody

• Featured Product
• KD/KO Validated

Pyruvate Carboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

41

Reviews (1)

Cat no : 16588-1-AP

Synonyms

PC, PCB, pyruvate carboxykinase, pyruvate Carboxylase, Pyruvic carboxylase

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Validation Data Gallery

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  WB analysis using 16588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16588-1-AP

  WB result of Pyruvate Carboxylase antibody (16588-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells.

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  IP experiment of HepG2 using 16588-1-AP

  IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:1000) with HepG2 cells lysate 2640 ug.

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  IP experiment of mouse liver using 16588-1-AP

  IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

• 

  IF Staining of HepG2 using 16588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, rat liver tissue
• Positive IP detected in: HepG2 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 34 publications below
• IHC: See 5 publications below
• IF: See 4 publications below
• IP: See 3 publications below

Product Information

16588-1-AP targets Pyruvate Carboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Pyruvate Carboxylase fusion protein Ag8857
• Full Name: pyruvate carboxylase
• Calculated Molecular Weight: 1178 aa, 130 kDa
• Observed Molecular Weight: 125-130 kDa
• GenBank Accession Number: BC011617
• Gene Symbol: Pyruvate Carboxylase
• Gene ID (NCBI): 5091
• RRID: AB_1851513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).

Publications

Species	Application	Title
human	WB	Nat Commun; Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells; Authors - Kevin M Rattigan
human	WB	Nat Commun; SARS-CoV-2 infection rewires host cell metabolism and is potentially susceptible to mTORC1 inhibition.; Authors - Peter J Mullen
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
human	IP	Nat Commun; The long noncoding RNA ADIPINT regulates human adipocyte metabolism via pyruvate carboxylase.; Authors - Alastair G Kerr
mouse	WB	Cancer Res; A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma.; Authors - Jennifer G Gill
mouse	WB	Elife; Differential requirements for mitochondrial electron transport chain components in the adult murine liver; Authors - Nicholas P Lesner

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kevin (Verified Customer) (02-10-2022)

This worked as well as other much more expensive antibodies from differenet suppliers

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562