Pyruvate Carboxylase Polyclonal antibody

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• Pyruvate Carboxylase Polyclonal antibody (16588-1-AP)

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• KD/KO Validated

Pyruvate Carboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16588-1-AP

Publications(70)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PC, EC:6.4.1.1, PCB, pyruvate carboxykinase, pyruvatecarboxykinase

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  WB analysis using 16588-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HepG2 using 16588-1-AP

  WB result of Pyruvate Carboxylase antibody (16588-1-AP; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Pyruvate Carboxylase transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HepG2 using 16588-1-AP

  IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:1000) with HepG2 cells lysate 2640 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of mouse liver using 16588-1-AP

  IP result of anti-Pyruvate Carboxylase (IP:16588-1-AP, 4ug; Detection:16588-1-AP 1:600) with mouse liver tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 16588-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16588-1-AP (Pyruvate Carboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin is stained using CL555-phalloidin (red) and DNA is stained by DAPI (blue).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 16588-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 16588-1-AP (Pyruvate Carboxylase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, rat liver tissue
• Positive IP detected in: HepG2 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16588-1-AP targets Pyruvate Carboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8857

  Product name: Recombinant human PC protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 828-1178 aa of BC011617

  Sequence: MERVFDYSEYWEGARGLYAAFDCTATMKSGNSDVYENEIPGGQYTNLHFQAHSMGLGSKFKEVKKAYVEANQMLGDLIKVTPSSKIVGDLAQFMVQNGLSRAEAEAQAEELSFPRSVVEFLQGYIGVPHGGFPEPFRSKVLKDLPRVEGRPGASLPPLDLQALEKELVDRHGEEVTPEDVLSAAMYPDVFAHFKDFTATFGPLDSLNTRLFLQGPKIAEEFEVELERGKTLHIKALAVSDLNRAGQRQVFFELNGQLRSILVKDTQAMKEMHFHPKALKDVKGQIGAPMPGKVIDIKVVAGAKVAKGQPLCVLSAMKMETVVTSPMEGTVRKVHVTKDMTLEGDDLILEIE

• Full Name: pyruvate carboxylase
• Calculated Molecular Weight: 1178 aa, 130 kDa
• Observed Molecular Weight: 125-130 kDa
• GenBank Accession Number: BC011617
• Gene Symbol: Pyruvate Carboxylase
• Gene ID (NCBI): 5091
• RRID: AB_1851513
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11498
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PC(pyruvate carboxylase) is a member of the family of biotin-dependent carboxylases and is found widely among eukaryotic tissues and in many prokaryotic species. It catalyses the ATP-dependent carboxylation of pyruvate to form oxaloacetate which may be utilised in the synthesis of glucose, fat, some amino acids or their derivatives and several neurotransmitters. Diabetes and hyperthyroidism increase the level of expression of pyruvate carboxylase in the long term, while its activity in the short term is controlled by the intramitochondrial concentrations of acetyl-CoA and pyruvate(PMID:9597748).

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; Mitochondrial control of glycerolipid synthesis by a PEP shuttle; Authors - Tadashi Yamamuro
human	WB	Cell Metab; Comprehensive isotopomer analysis of glutamate and aspartate in small tissue samples; Authors - Feng Cai ; KD Validated
		Nat Metab; Redox-dependent liver gluconeogenesis impacts different intensity exercise in mice; Authors - Takahiro Horiuchi
mouse	WB	Cancer Res; A short isoform of spermatogenic enzyme GAPDHS functions as a metabolic switch and limits metastasis in melanoma.; Authors - Jennifer G Gill
mouse	WB	Nat Commun; Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise.; Authors - Jin-Ho Koh
mouse	WB	Nat Commun; MCT1-governed pyruvate metabolism is essential for antibody class-switch recombination through H3K27 acetylation; Authors - Wenna Chi

Reviews

5.0 out of 5

2 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Amy (Verified Customer) (02-06-2026)

Brilliant antibody. Had overexpressed PC in my cells and saw a nice strong band

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mammary gland tumour cells

Kevin (Verified Customer) (02-10-2022)

This worked as well as other much more expensive antibodies from differenet suppliers

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: K562