SCNN1A Polyklonaler Antikörper

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• KD/KO Validated

SCNN1A Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 10924-2-AP

Synonyme

Alpha ENaC, Alpha NaCH, ENaCa, ENaCalpha, SCNEA, SCNN1, SCNN1A

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10924-2-AP

  WB result of SCNN1A (10924-2-AP, 1:1000) with si-control and si-SCNN1A transfected HEK293 cells.

• 

  WB analysis of PC-13 using 10924-2-AP

  PC-13 cells were subjected to SDS PAGE followed by western blot with 10924-2-AP (SCNN1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10924-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10924-2-AP (SCNN1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10924-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10924-2-AP (SCNN1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 10924-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10924-2-AP (SCNN1A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10924-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10924-2-AP (SCNN1A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 10924-2-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 10924-2-AP (SCNN1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-13-Zellen, HEK-293
• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 7 publications below

Produktinformation

10924-2-AP bindet in WB, IF, IHC, ELISA SCNN1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCNN1A fusion protein Ag1325
• Vollständiger Name: sodium channel, nonvoltage-gated 1 alpha
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC006526
• Gene symbol: SCNN1A
• Gene ID (NCBI): 6337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCNN1A (sodium channel, non-voltage-gated 1 alpha), also known as ENaCA (epithelial Na(+) channel subunit alpha) or amiloride-sensitive sodium channel subunit alpha, is the alpha subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. ENaC consists of three different subunits: alpha, beta, gamma. Mutations in the gene of SCNN1A have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It has been reported that full-length SCNN1A protein can be cleaved into 65- and 30-kDa fragments (PMID: 16477034; 18701608).

Publikationen

Species	Application	Title
human	WB	Am J Respir Crit Care Med; SPX-101 is a Novel ENaC-targeted Therapeutic for Cystic Fibrosis that Restores Mucus Transport.; Authors - David W Scott
mouse	IHC	Sci Adv; MLL4 mediates differentiation and tumor suppression through ferroptosis.; Authors - Shaun Egolf
mouse	WB	Hypertension; Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.; Authors - Shuai Shao
	IHC	Sci Rep; Distribution of aquaporins and sodium transporters in the gastrointestinal tract of a desert hare, Lepus yarkandensis.; Authors - Jianping Zhang
human	WB	Biomed Pharmacother; PMID: 31203134; Authors - Wei-Hua He
human	WB,IHC	PLoS One; Reduced Expression of Enac in Placenta Tissues of Patients with Severe Preeclampsia Is Related to Compromised Trophoblastic Cell Migration and Invasion during Pregnancy.; Authors - Wang Shan S

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