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SARS-CoV-2 Spike Rekombinanter Antikörper

SARS-CoV-2 Spike Rekombinant Antikörper für Neutralization, ELISA

Wirt / Isotyp

Mensch / IgG1

Getestete Reaktivität

Virus

Anwendung

Neutralization, ELISA

Konjugation

Unkonjugiert

CloneNo.

AM001414

Kat-Nr. : 91361-PTG

Synonyme

SARS-CoV-2, SARS-CoV-2 Spike, Spike

Galerie der Validierungsdaten

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  • Neutralization experiment of SARS-CoV-2 Spike using SARS-CoV-2 Spike Recombinant antibody (91361-PTG)

    Neutralization experiment of SARS-CoV-2 Spike using 91361-PTG

    SARS-CoV-2 Spike Antibody (clone AM001414) tested by Neutralization.Viral neutralization assays were performed with pseudotyped virus carrying a luciferase reporter gene and bearing the SARS-CoV-2 S1 spike glycoprotein. A549 lung epithelial target cells expressing the ACE2 receptor were incubated with virus and a graded dose of SARS-CoV-2 Spike Antibody (clone AM001414). Luciferase signal, indicative of cellular infection and viral gene expression, was measured. Viral neutralization by SARS-CoV-2 antibodies inhibits viral entry and, by extension, virus associated luciferase signal in manner proportional to antibody dose. Data provided by Active Motif®. .

  • ELISA experiment of SARS-CoV-2 Spike RBD protein using SARS-CoV-2 Spike Recombinant antibody (91361-PTG)

    ELISA experiment of SARS-CoV-2 Spike RBD protein using 91361-PTG

    SARS-CoV-2 Spike Antibody (clone AM001414) tested by ELISA.SARS-CoV-2 Spike RBD protein was coated onto microtiter plates at 0.5 µg/mL and then incubated with a dilution series of SARS-CoV-2 Spike Antibody (clone AM009105). Bound antibodies were detected with anti-human IgG conjugated to horseradish peroxidase (HRP) followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm. Data provided by Active Motif®. .

Geprüfte Anwendungen

Erfolgreiche NeutralizationPseudotypisiertes Virus
Erfolgreiche Detektion in ELISASARS-CoV-2-Spike-RBD-Protein

Empfohlene Verdünnung

AnwendungVerdünnung
Sample dependent. To be determined by the end user.

Note: Proteintech is partnering with Active Motif to bring these cutting-edge, patient derived antibodies to our customers during this important time for COVID-19 research.

Produktinformation

91361-PTG bindet in Neutralization, ELISA SARS-CoV-2 Spike und zeigt Reaktivität mit Virus

Getestete Reaktivität Virus
Wirt / Isotyp Mensch / IgG1
Klonalität Rekombinant
Typ Antikörper
Immunogen Virus
Vollständiger Name SARS-CoV-2 Spike Protein
Berechnetes Molekulargewicht 141 kDa
GenBank-ZugangsnummerNC_045512
Gene symbol COVID-19 S Protein
Gene ID (NCBI) 43740568
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Chromatographie
Lagerungspuffer 140 mM Hepes, pH 7,5, 70 mM NaCl, 32 mM NaOAc, 0,035% Natriumazid und 30% Glyzerin.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COVID-19, which is short for coronavirus disease 2019, is the official name of the respiratory disease caused by infection with the novel coronavirus SARS-CoV-2. The virus that causes COVID-19 was named SARS-CoV-2 because it is a coronavirus genetically similar to, yet distinct from, the virus that caused the severe acute respiratory syndrome (SARS) outbreak in 2003. Studying the details of how this virus replicates and causes the disease will allow scientists and physicians to more rapidly develop fast and accurate methods of detection as well as to deploy therapeutic and vaccine strategies. This antibody was derived from COVID-19 patients who have cleared the virus. Patient serum IgG was sequenced and expressed as full-length IgG1 with human immunoglobulin heavy and light chains in mammalian 293 cells.