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ACE2 Polyclonal antibody
ACE2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 21115-1-AP
Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, human skeletal muscle tissue|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human kidney tissue, mouse testis tissue, human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human kidney tissue, mouse kidney tissue|
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
21115-1-AP targets ACE2 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ACE2 fusion protein Ag15455|
|Full Name||angiotensin I converting enzyme (peptidyl-dipeptidase A) 2|
|Calculated molecular weight||805 aa, 92 kDa|
|Observed molecular weight||120 kDa, 92 kDa|
|GenBank accession number||BC048094|
|Gene ID (NCBI)||59272|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).
|Product Specific Protocols|
|WB protocol for ACE2 antibody 21115-1-AP||Download protocol|
|IHC protocol for ACE2 antibody 21115-1-AP||Download protocol|
|IF protocol for ACE2 antibody 21115-1-AP||Download protocol|
|IP protocol for ACE2 antibody 21115-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.
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Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Magdalena (Verified Customer) (07-18-2022)
I'm satisfied with this antibody. I used this antibody in dot-blot technique and IF. In IF I used dilution factor 1:200, seconday antibody was alexa fluor 555.
Ratna (Verified Customer) (01-14-2021)