ACE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ACE2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

128

Reviews (2)

Cat no : 21115-1-AP

Synonyms

Angiotensin-converting enzyme homolog, Angiotensin-converting enzyme 2, Angiotensin converting enzyme 2, ACE-related carboxypeptidase, ACEH

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Validation Data Gallery

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  WB analysis using 21115-1-AP

  mouse kidney tissue lysates were subjected to SDS PAGE followed by western blot with 21115-1-AP (ACE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 21115-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21115-1-AP (ACE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 21115-1-AP

  IP result of anti-ACE2 (IP:21115-1-AP, 5ug; Detection:21115-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 21115-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21115-1-AP (ACE2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

• 

  IF Staining of human kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), PTPRO antibody (67000-1-Ig, Clone: 2F2B4, red).

• 

  IF Staining of mouse kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 21115-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using ACE2 antibody (21115-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, human skeletal muscle tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human kidney tissue, mouse testis tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human kidney tissue, mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 92 publications below
• IHC: See 24 publications below
• IF: See 31 publications below
• IP: See 3 publications below
• FC: See 8 publications below
• CoIP: See 3 publications below

Product Information

21115-1-AP targets ACE2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACE2 fusion protein Ag15455
• Full Name: angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• Calculated Molecular Weight: 805 aa, 92 kDa
• Observed Molecular Weight: 120 kDa, 92 kDa
• GenBank Accession Number: BC048094
• Gene Symbol: ACE2
• Gene ID (NCBI): 59272
• RRID: AB_10732845
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACE2 (Angiotensin-converting enzyme 2), also named as ACEH, is a zinc metalloprotease of the ACE family and a critical regulator of the reninangiotensin system. ACE2 has a more restricted tissue distribution than ACE, being found predominantly in the heart, kidneys, and testes although low levels have been detected in a variety of tissues (PMID:15983030). ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (PMID: 32142651). The expression level and expression pattern of human ACE2 in different tissues might be critical for the susceptibility, symptoms, and outcome of 2019-nCoV/SARS-CoV-2 infection (PMID: 32133153). It can be used as a potential therapeutic target of SARS-CoV-2 (PMID: 32125455). The calculated molecular weight of ACE2 is 92kDa but it migrates to 120kDa due to N-glycosylation (PMID:16166094). Sometimes, several cleaved fragments can also be detected as 75kDa, 50 kDa or 37kDa (PMID: 29561187, 22009550, 30759273). It has 2 isoforms produced by alternative splicing. This antibody is specific to ACE2. The location of ACE2 is membrane and cytoplasm, however it accumulates in the nucleus during the mitosis (PMID: 1730413/PMID: 18292088).

Publications

Species	Application	Title
human	WB	Nat Genet; Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs; Authors - Antoine Rebendenne
human	IF	Cell Stem Cell; Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men.; Authors - Ryan M Samuel
human,mouse	IF	Cell Res; Receptome profiling identifies KREMEN1 and ASGR1 as alternative functional receptors of SARS-CoV-2.; Authors - Yunqing Gu
human	IHC	Nat Neurosci; Olfactory transmucosal SARS-CoV-2 invasion as a port of central nervous system entry in individuals with COVID-19.; Authors - Jenny Meinhardt
human	WB,FC	Mol Cell; Enhanced SARS-CoV-2 entry via UPR-dependent AMPK-related kinase NUAK2; Authors - Vibhu Prasad
human	WB	Eur Respir J; Influenza virus infection increases ACE2 expression and shedding in human small airway epithelial cells.; Authors - Kelly S Schweitzer

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Magdalena (Verified Customer) (07-18-2022)

I'm satisfied with this antibody. I used this antibody in dot-blot technique and IF. In IF I used dilution factor 1:200, seconday antibody was alexa fluor 555.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:200

FH

Ratna (Verified Customer) (01-14-2021)

Good

• Applications: Western Blot