RUFY1 Polyklonaler Antikörper

RUFY1 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 13498-1-AP

Synonyme

RUN and FYVE domain-containing protein 1, RUFY 1, RABIP4, Rab4-interacting protein, La-binding protein 1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13498-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13498-1-AP (RUFY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa cells using 13498-1-AP

  IP result of anti-RUFY1 (13498-1-AP for IP and Detection).

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  IF Staining of HeLa using 13498-1-AP

  Immunofluorescent analysis of Hela cells, using RUFY1 antibody 13498-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

13498-1-AP bindet in WB, IF/ICC, IP, CoIP, ELISA RUFY1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RUFY1 fusion protein Ag4324
• Vollständiger Name: RUN and FYVE domain containing 1
• Berechnetes Molekulargewicht: 708 aa, 80 kDa
• Beobachtetes Molekulargewicht: 69 kDa, 80 kDa
• GenBank-Zugangsnummer: BC032571
• Gene symbol: RUFY1
• Gene ID (NCBI): 80230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab proteins form the largest group within the Ras superfamily of small GTPases, which acts as molecular switches by cycling between their inactive active GTP-bound and GDP-bound forms [PMID:15520808]. The small GTP-binding protein Rab4 has been involved in the recycling of αvβ3 integrins in response to platelet-derived growth factor (PDGF) stimulation suggesting a role for Rab4 in cell adhesion and migration [PMID:17001082]. Rabip4 (Rab4 interacting protein), which possesses a RUN domain, two coiled-coil domains and a FYVE finger, was identified as an effector of Rab4, a small GTPase that plays a role in the endocytotic pathway in all cell types [PMID:11172003]. RUFY1 exsits three isoforms and molecular weight of isoforms are 80 kDa,69 kDa and 45 kDa.

Publikationen

Species	Application	Title
mouse	WB,IF	Cell; Mouse oocytes sequester aggregated proteins in degradative super-organelles; Authors - Gabriele Zaffagnini
human	IF	Nat Commun; Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5.; Authors - Meng Shi
mouse	IF	Mol Biol Cell; Trafficking defects in WASH-knockout fibroblasts originate from collapsed endosomal and lysosomal networks.; Authors - Gomez Timothy S TS
human	WB, IF	Mol Biol Cell; COMMD1 is linked to the WASH complex and regulates endosomal trafficking of the copper transporter ATP7A.; Authors - Christine A Phillips-Krawczak
human	CoIP	Cancer Sci; Podocalyxin-like protein promotes gastric cancer progression through interacting with RUN and FYVE domain containing 1 protein.; Authors - Qiaoming Zhi