RSPO3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RSPO3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 17193-1-AP

Synonyme

CRISTIN1, hPWTSR, hRspo3, Protein with TSP type 1 repeat, PWTSR, R spondin 3, Roof plate specific spondin 3, RSPO3, THSD2

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 17193-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 17193-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 17193-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 17193-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 17193-1-AP (RSPO3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 17193-1-AP

  IP Result of anti-RSPO3 (IP:17193-1-AP, 4ug; Detection:17193-1-AP 1:300) with human placenta tissue lysate 2800ug.

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human placenta using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human placenta using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 17193-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17193-1-AP (RSPO3 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Plazenta-Gewebe, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

17193-1-AP bindet in WB, IP, IHC, IF, ELISA RSPO3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RSPO3 fusion protein Ag9899
• Vollständiger Name: R-spondin 3 homolog (Xenopus laevis)
• Berechnetes Molekulargewicht: 272 aa, 31 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC022367
• Gene symbol: RSPO3
• Gene ID (NCBI): 84870
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RSPO3, also named as R-spondin-3, is a 272 amino acid protein, which contains 1 TSP type-1 domain and 2 FU (furin-like) repeats. RSPO3 may be a secreted protein and belongs to the R-spondin family. RSPO3 is expressed at higher level in placenta, small intestine, fetal thymus and lymph node. RSPO3 is an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. The four R-spondins (RSPO1-4) and three related leucinerich repeat-containing G-protein coupled receptors, LGR4, LGR5, and LGR6 (LGR4-6), constitute a ligand-receptor system that plays critical roles in development, stem cell survival, and oncogenesis. Ectopic expression of RSPO2/3in mouse mammary epithelial cells led to an increase in invasiveness in vitro and in tumor formation and metastasis in vivo.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; R-Spondin chromosome rearrangements drive Wnt-dependent tumour initiation and maintenance in the intestine.; Authors - Teng Han
human	WB	Proc Natl Acad Sci U S A; RSPO-LGR4 functions via IQGAP1 to potentiate Wnt signaling.; Authors - Kendra S Carmon
mouse	WB,IF	Cell Death Dis; Endothelial cell-derived RSPO3 activates Gαi1/3-Erk signaling and protects neurons from ischemia/reperfusion injury; Authors - Ting-Tao Liu ; KD Validated
human	WB	Cardiovasc Res; Role of R-spondin 2 in arterial lymphangiogenesis and atherosclerosis.; Authors - Bhupesh Singla
human	IHC	Am J Physiol Cell Physiol; R-Spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.; Authors - Zhilong Chen
human	IHC	J Exp Clin Cancer Res; Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses.; Authors - Tomohiko Shinkawa

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