ROR1 Polyklonaler Antikörper

ROR1 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 20629-1-AP

Synonyme

NTRKR1, ROR1

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Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 20629-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20629-1-AP (ROR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20629-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20629-1-AP (ROR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 20629-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 20629-1-AP (ROR1 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Produktinformation

20629-1-AP bindet in WB, IHC, IF, ELISA ROR1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ROR1 fusion protein Ag14586
• Vollständiger Name: receptor tyrosine kinase-like orphan receptor 1
• Berechnetes Molekulargewicht: 937 aa, 104 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC006374
• Gene symbol: ROR1
• Gene ID (NCBI): 4919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROR1 is a member of the RTK family of orphan receptors related to muscle specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937-amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

Publikationen

Species	Application	Title
human	WB	Oncol Rep; Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells.; Authors - Bin Dai
human	IHC	Virchows Arch; Expression of ROR1 has prognostic significance in triple negative breast cancer.; Authors - Hui-Ping Chien
human	IHC	Biomedicines; ROR1 Is Expressed in Diffuse Large B-Cell Lymphoma (DLBCL) and a Small Molecule Inhibitor of ROR1 (KAN0441571C) Induced Apoptosis of Lymphoma Cells.; Authors - Amineh Ghaderi
human	WB	Cancer Manag Res; Wnt5a induces ROR1 and ROR2 to activate RhoA in esophageal squamous cell carcinoma cells.; Authors - Xuping Wu
rat	IF	Am J Physiol Cell Physiol; Periodontal Ligaments Enhance Neurite Outgrowth in Trigeminal Ganglion Neurons through Wnt5a Production Induced by Mechanical Stimulation; Authors - Kaori Takahashi
human	IHC	Pharmaceutics; A Small Molecule Targeting the Intracellular Tyrosine Kinase Domain of ROR1 (KAN0441571C) Induced Significant Apoptosis of Non-Small Cell Lung Cancer (NSCLC) Cells; Authors - Amineh Ghaderi

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