TTF1 Monoclonal antibody

TTF1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

2C8F3

Cat no : 66034-1-Ig

Synonyms

BHC, Homeobox protein Nkx 2.1, NK 2, NK2 homeobox 1, NKX2 1, NKX2.1, NKX2-1, NKX2A, TEBP, Thyroid nuclear factor 1, Thyroid transcription factor 1, TITF1, TTF 1, TTF1

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Validation Data Gallery

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  WB analysis of HepG2 using 66034-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66034-1-Ig (TTF1 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human thyroid cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 66034-1-Ig(NKX2-1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human thyroid cancer using 66034-1-Ig

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66034-1-Ig (TTF1 antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 66034-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using TTF1 antibody (66034-1-Ig, Clone: 2C8F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human thyroid cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human lung cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

66034-1-Ig targets TTF1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NK2 homeobox 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: NM_003317
• Gene Symbol: TTF1
• Gene ID (NCBI): 7080
• RRID: AB_11045663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homeobox protein Nkx-2.1 (NKX2-1), also named Thyroid nuclear factor 1,Thyroid transcription factor 1(TTF1). It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD).

Publications

Species	Application	Title
human	WB	ACS Omega; Interpreting the Pharmacological Mechanisms of Sho-saiko-to on Thyroid Carcinoma through Combining Network Pharmacology and Experimental Evaluation.; Authors - Kun Wang
human	WB	Cell Biochem Biophys; Suberoylanilide Hydroxamic Acid (SAHA) Reduces Fibrosis Markers and Deactivates Human Stellate Cells via the Epithelial-Mesenchymal Transition (EMT).; Authors - Merve Özel
human	IHC	Nat Commun; Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features; Authors - Weizhu Zhao