RAB3GAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB3GAP1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (2)

Kat-Nr. : 21663-1-AP

Synonyme

KIAA0066, P130, Rab3 GAP, Rab3 GAP p130, RAB3 GTPase-activating protein 1, RAB3GAP, RAB3GAP1, RAB3GAP130, WARBM1

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Galerie der Validierungsdaten

• 

  WB analysis using 21663-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 21663-1-AP

  WB result of RAB3GAP1 antibody (21663-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB3GAP1 transfected HEK-293 cells.

• 

  IHC staining of human testis using 21663-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21663-1-AP (RAB3GAP1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 21663-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAB3GAP1 antibody 21663-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HL-60-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

21663-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA RAB3GAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB3GAP1 fusion protein Ag16426
• Vollständiger Name: RAB3 GTPase activating protein subunit 1 (catalytic)
• Berechnetes Molekulargewicht: 981 aa, 111 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC146809
• Gene symbol: RAB3GAP1
• Gene ID (NCBI): 22930
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB3GAP1 (RAB3 GTPase-activating protein 1) is the catalytic subunit of the heterodimeric enzyme RAB3GAP (RAB3GTPase-activating protein), a key regulator of the RAB3 cycle, which controls calcium-mediated exocytosis of neurotransmitters and hormones. Mutations in RAB3GAP1 cause Warburg Micro syndrome (OMIM 600118) and Martsolf syndrome (OMIM 212720), phenotypically overlapping autosomal recessive conditions characterized by ocular and neurodevelopmental manifestations.

Publikationen

Species	Application	Title
monkey	WB	J Cell Biol; Rab18 and a Rab18 GEF complex are required for normal ER structure.; Authors - Andreas Gerondopoulos ; KD Validated
human	WB	EMBO J; COPI-TRAPPII activates Rab18 and regulates its lipid droplet association.; Authors - Chunman Li
human, mouse	WB	BMC Biol; VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase.; Authors - Yorann Baron
mouse	WB	J Neurosci; Mutant Huntingtin Impairs BDNF Release from Astrocytes by Disrupting Conversion of Rab3a-GTP into Rab3a-GDP.; Authors - Yan Hong
rat	WB,IF	Toxicol Lett; Manganese-induced alpha-synuclein overexpression impairs synaptic vesicle fusion by disrupting the Rab3 cycle in primary cultured neurons.; Authors - Tong-Yu Wang
human	WB	Open Biol; Warburg Micro syndrome is caused by RAB18 deficiency or dysregulation.; Authors - Mark T Handley

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sammy (Verified Customer) (11-05-2023)

Detects a band at the correct MW via western blotting. An unspecific band also detected around 40 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HUVEC

RAB3GAP1 Antibody Western Blot validation (1:1000 dilution) in HUVEC (Cat no:21663-1-AP)

FH

Bing (Verified Customer) (08-04-2021)

OK to probe RAB3GAP1, but sometimes produces an unspecific band nearby. 66688-1-Ig seems better.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mouse brain lysate

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