QSOX1 Polyklonaler Antikörper

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• KD/KO Validated

QSOX1 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 12713-1-AP

Synonyme

UNQ2520/PRO6013, QSCN6, Q6, hQSOX, EC:1.8.3.2

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Galerie der Validierungsdaten

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  WB analysis using 12713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12713-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12713-1-AP (QSOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of SW 1990 using 12713-1-AP

  IP result of anti-QSOX1 (IP:12713-1-AP, 4ug; Detection:12713-1-AP 1:300) with SW 1990 cells lysate 2400ug.

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  IHC staining of human liver cancer using 12713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12713-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12713-1-AP (QSOX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 12713-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 12713-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using QSOX1 antibody (12713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SW 1990-Zellen, humanes Plazenta-Gewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: SW 1990-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 8 publications below
• IF: See 3 publications below

Produktinformation

12713-1-AP bindet in WB, IHC, IF-P, IP, ELISA QSOX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: QSOX1 fusion protein Ag3396
• Vollständiger Name: quiescin Q6 sulfhydryl oxidase 1
• Berechnetes Molekulargewicht: 747 aa, 83 kDa
• Beobachtetes Molekulargewicht: 66-83kDa
• GenBank-Zugangsnummer: BC017692
• Gene symbol: QSOX1
• Gene ID (NCBI): 5768
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

QSOX1(Sulfhydryl oxidase 1) catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide.It has two isoforms with the molecular weight of 67 kda and 83 kDa,respectively. It is detected a band of 95 Kda in HT1080 cell lines (PMID:17331072).Isoform 1 is expressed on golgi apparatus membrane and isoform 2 is secreted (PMID:10708601). The isoform1(QSOX1a) is the full length of QSOX1, approximately 78 kd; and the isoform2(QSOX1b) is the splicing form, approximately 62kd(PMID:14633699; 2496424). QSOX1 is highly expressed in tumor cell lines but is not expressed in adjacent normal cells (PMID:21989104).

Publikationen

Species	Application	Title
human	IHC	Redox Biol; Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activation.; Authors - Jialei Sun
	WB	Antioxid Redox Signal; The Emerging Role of QSOX1 in Cancer.; Authors - Douglas F Lake
human	IHC	Breast Cancer Res; High expression of QSOX1 reduces tumorogenesis, and is associated with a better outcome for breast cancer patients.; Authors - Pernodet Nicolas N
human	WB,IHC	Breast Cancer Res; Expression of quiescin sulfhydryl oxidase 1 is associated with a highly invasive phenotype and correlates with a poor prognosis in Luminal B breast cancer.; Authors - Katchman Benjamin A BA
human	IHC	Mod Pathol; QSOX1 expression is associated with aggressive tumor features and reduced survival in breast carcinomas.; Authors - Gøril Knutsvik
human	IHC	Oncotarget; Reduced QSOX1 enhances radioresistance in nasopharyngeal carcinoma.; Authors - Lei Zhou

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