Liprin Alpha 1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Liprin Alpha 1 Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

25

Kat-Nr. : 14175-1-AP

Synonyme

LAR interacting protein 1, LIP 1, LIP.1, LIP1, LIPRIN, Liprin alpha 1, PPFIA1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 14175-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 14175-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 14175-1-AP

  IP Result of anti-Liprin alpha 1 (IP:14175-1-AP, 4ug; Detection:14175-1-AP 1:500) with MCF-7 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14175-1-AP (Liprin alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14175-1-AP

  Immunofluorescent analysis of HepG2 cells, using PPFIA1 antibody 14175-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HeLa using 14175-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Liprin alpha 1 antibody (14175-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 17 publications below

Produktinformation

14175-1-AP bindet in WB, IP, IHC, IF, FC, ELISA Liprin Alpha 1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Liprin Alpha 1 fusion protein Ag5381
• Vollständiger Name: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1
• Berechnetes Molekulargewicht: 1202 aa, 136 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC034046
• Gene symbol: PPFIA1
• Gene ID (NCBI): 8500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.

Publikationen

Species	Application	Title
human	IF	Nat Commun; Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity.; Authors - Xingqiao Xie
human	WB,IF	Nat Commun; PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.; Authors - Giulia Mana ; KD Validated
mouse	IF	Elife; Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion.; Authors - Dillon Jevon
human	IF	Stem Cells Transl Med; Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix.; Authors - Reena Singh
human	WB	J Cell Sci; Liprin-α1, ERC1 and LL5 identify a polarized, dynamic compartment implicated in cell migration.; Authors - Veronica Astro ; KD Validated
human	IF	Open Biol; The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions; Authors - Xingchen Li

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