PMEPA1 Polyklonaler Antikörper

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• KD/KO Validated

PMEPA1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16521-1-AP

Synonyme

PMEPA1, STAG1, TMEPAI

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Galerie der Validierungsdaten

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  WB analysis using 16521-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16521-1-AP (PMEPA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 16521-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16521-1-AP (PMEPA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-3 using 16521-1-AP

  Immunofluorescent analysis of PC-3 cells using 16521-1-AP (PMEPA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16521-1-AP bindet in WB, IHC, IF, ELISA PMEPA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PMEPA1 fusion protein Ag9744
• Vollständiger Name: prostate transmembrane protein, androgen induced 1
• Berechnetes Molekulargewicht: 287 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32-36 kDa
• GenBank-Zugangsnummer: BC015918
• Gene symbol: PMEPA1
• Gene ID (NCBI): 56937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PMEPA1, also known as TMEPAI, was first discovered as androgen inducible gene in hormone responsive LNCaP cells. PMEPA1 is highly expressed in various solid tumors and is known to play important roles in the transforming growth factor-β(TGF-β) signaling pathway (PMID: 27697531).

Publikationen

Species	Application	Title
human	WB	EMBO Mol Med; An autocrine ActivinB mechanism drives TGFβ/Activin signaling in Group 3 medulloblastoma.; Authors - Morgane Morabito
human	WB,IHC	Front Immunol; PMEPA1 Is a Prognostic Biomarker That Correlates With Cell Malignancy and the Tumor Microenvironment in Bladder Cancer; Authors - Dongxu Qiu ; KD Validated
mouse	IF	FASEB J; PMEPA1 and NEDD4 control the proton production of osteoclasts by regulating vesicular trafficking.; Authors - Hirohito Hirata ; KO Validated
human	WB	J Virol; NF-κB-Interacting Long Noncoding RNA Regulates HIV-1 Replication and Latency by Repressing NF-κB Signaling.; Authors - Hong Wang
human	WB	Biochem Biophys Res Commun; PMEPA1, a TGF-β- and hypoxia-inducible gene that participates in hypoxic gene expression networks in solid tumors.; Authors - Masaru Koido ; KD Validated
human	WB	Genes Cancer; Increased Smad3 and reduced Smad2 levels mediate the functional switch of TGF-β from growth suppressor to growth and metastasis promoter through TMEPAI/PMEPA1 in triple negative breast cancer.; Authors - Prajjal K Singha

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