PIP4K2A Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PIP4K2A Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12469-1-AP

Synonyme

Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, Phosphatidylinositol 5-Phosphate 4-Kinase, EC:2.7.1.149, Diphosphoinositide kinase 2-alpha

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 12469-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12469-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12469-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12469-1-AP (PIP4K2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12469-1-AP

  IP result of anti-PIP4K2A (IP:12469-1-AP, 4ug; Detection:12469-1-AP 1:500) with mouse brain tissue lysate 4400ug.

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  IHC staining of human spleen using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human spleen using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 12469-1-AP (PIP4K2A Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12469-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12469-1-AP (PIP4K2A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 12469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 12469-1-AP (PIP4K2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12469-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PIP4K2A antibody (12469-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (black).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HeLa-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Milzgewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

12469-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA PIP4K2A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PIP4K2A fusion protein Ag3152
• Vollständiger Name: phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
• Berechnetes Molekulargewicht: 406 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC018034
• Gene symbol: PIP4K2A
• Gene ID (NCBI): 5305
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,human	IF,IHC	Dev Cell; PI5P4Ks drive metabolic homeostasis through peroxisome-mitochondria interplay.; Authors - Archna Ravi
human	IF,IHC,IP,WB	Int J Biochem Cell Biol; A novel lncRNA lncSAMD11-1:1 interacts with PIP4K2A to promote endometrial decidualization by stabilizing FoxO1 nuclear localization; Authors - Mei Zhang
human,mouse	WB,IHC,IF	Sci Adv; PI5P4Kα supports prostate cancer metabolism and exposes a survival vulnerability during androgen receptor inhibition; Authors - Joanna Triscott ; KD Validated